Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry

Scientific Reports
Naila Irum HadiSyed Ghulam Musharraf

Abstract

Detection of metabolic signature for breast cancer (BC) has the potential to improve patient prognosis. This study identified potentially significant metabolites differentiating between breast cancer patients and healthy controls to help in diagnosis, grading, staging and determination of neoadjuvant status. Serum was collected from 152 pre-operative breast cancer (BC) patients and 155 healthy controls in this case-controlled study. Gas chromatography-mass spectrometry (GC-MS) was used to obtain metabolic profiles followed by chemometric analysis with the identification of significantly differentiated metabolites including 7 for diagnosis, 18 for grading, 23 for staging, 15 for stage III subcategory and 10 for neoadjuvant status (p-value < 0.05). Partial Least Square Discriminant Analysis (PLS-DA) model revealed a distinct separation between healthy controls and BC patients with a sensitivity of 96% and specificity of 100% on external validation. Models for grading, staging and neoadjuvant status were built with Decision Tree Algorithm with predictive accuracy of 71.5%, 71.3% and 79.8% respectively. Pathway analysis revealed increased glycolysis, lipogenesis, and production of volatile organic metabolites indicating the metabol...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E Bustamante, P L Pedersen
Aug 1, 1994·Free Radical Biology & Medicine·C M KneepkensC C Roy
Sep 14, 2000·The Journal of Laboratory and Clinical Medicine·M PhillipsF Rahbari-Oskoui
May 20, 2003·The Breast Journal·Michael PhillipsCynthia Wong
Mar 6, 2004·Expert Review of Molecular Diagnostics·John C LindonJeremy K Nicholson
Nov 25, 2004·Technology in Cancer Research & Treatment·Cynthia LeanCarolyn Mountford
Oct 18, 2005·NMR in Biomedicine·Beathe SitterIngrid S Gribbestad
Sep 19, 2006·Biochimica Et Biophysica Acta·Frank HannemannRita Bernhardt
Oct 19, 2006·Current Opinion in Cell Biology·Reuben J Shaw
Oct 25, 2006·Breast Cancer Research and Treatment·Tone F BathenSteinar Lundgren
Aug 8, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S J LordN Houssami
Apr 29, 2008·Molecular Cell·Dana M GwinnReuben J Shaw
Dec 10, 2009·Journal of Proteome Research·Guro F GiskeødegårdTone F Bathen
Apr 21, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M Elizabeth H HammondAntonio C Wolff
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Frank WeinbergNavdeep S Chandel
May 13, 2010·The International Journal of Biochemistry & Cell Biology·Katarína SmolkováPetr Ježek
Jul 22, 2010·Cancer Research·Haibo LiuAnthony P Heaney
Sep 14, 2010·BMC Medical Genomics·Gunnar SchrammRainer König
Oct 20, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carolyn M SlupskyMichael B Sawyer
Oct 21, 2010·Cancer Research·Vincent M AsiagoDaniel Raftery
Mar 9, 2011·Journal of Breath Research·Michael PhillipsJames Wai
Mar 1, 2013·Nature Reviews. Cancer·Arkaitz CarracedoPier Paolo Pandolfi
Aug 27, 2014·International Journal of Cancer. Journal International Du Cancer·Jan BudcziesCarsten Denkert
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray

❮ Previous
Next ❯

Citations

Feb 22, 2018·International Journal of Molecular Sciences·Marcella Regina CardosoSophie Françoise Mauricette Derchain
May 17, 2019·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Xingzhi ZhouShuangping Liu
Sep 9, 2020·Analytical and Bioanalytical Chemistry·Gustavo Henrique Bueno DuarteAna Valéria Colnaghi Simionato
Oct 1, 2020·Cancers·Jiawei LiDong-Xu Liu
May 3, 2019·Cancer & Metabolism·Matthias PietzkeUNKNOWN METTEN study group
Aug 16, 2019·Expert Review of Molecular Diagnostics·Pro Kit CheungSze Chuen Cesar Wong
Aug 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danielly C Ferraz da CostaEliane Fialho
Feb 17, 2019·Breast Cancer Research and Treatment·Hua ZhaoWong-Ho Chow
Jul 11, 2019·Molecular and Clinical Oncology·Nurul Azmir Amir HashimMusalmah Mazlan
Oct 8, 2020·Critical Reviews in Analytical Chemistry·M Fraga-CorralJ Simal-Gandara
Mar 5, 2021·Pharmacology & Therapeutics·Lingfan LiangZeping Hu
May 4, 2021·Frontiers in Cell and Developmental Biology·Xiaodan HouQiong Li
May 25, 2021·Bioengineering & Translational Medicine·Kookrae ChoEunjoo Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
Nuclear magnetic resonance
Profiler
NMR
PCA
biopsies

Software Mentioned

Mass Profiler Professional ( MPP )
Metaboanalyst
DA
PLS
MPP
Mass Profiler Professional
Agilent Mass Hunter Qualitative Analysis

Related Concepts

Related Papers

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Hu SongBi-Yan Lu
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Shao LiuLijian Tao
© 2022 Meta ULC. All rights reserved