Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus

Frontiers in Microbiology
Wenjie GongChangchun Tu

Abstract

Classical swine fever (CSF) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide. The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of CSF. Birefly, serum metabolomics of CSFV Shimen strain-infected piglets were analyzed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) in combination with multivariate statistical analysis. In CSFV-infected piglets at days 3 and 7 post-infection changes were found in metabolites associated with several key metabolic pathways, including tryptophan catabolism and the kynurenine pathway, phenylalanine metabolism, fatty acid and lipid metabolism, the tricarboxylic acid and urea cycles, branched-chain amino acid metabolism, and nucleotide metabolism. Several pathways involved in energy metabolism including fatty acid biosynthesis and β-oxidation, branched-chain amino acid metabolism, and the tricarboxylic acid cycle were significantly inhibited. Changes were also observed in several metabolites exclusively associated with gut microbiota. The metabolomic profiles indicate that CSFV-host gut microbiome interac...Continue Reading

References

May 1, 1976·Somatic Cell Genetics·C Jones, E E Moore
Apr 23, 2002·Kidney International·Tsuneo DeguchiTetsuya Terasaki
Sep 17, 2002·Cell Death and Differentiation·F FallarinoP Puccetti
Mar 6, 2003·The Veterinary Journal·V MoennigI Greiser-Wilke
Jul 17, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·G Floegel-NiesmannV Moennig
Dec 21, 2005·The Journal of Nutrition·Yoshiharu ShimomuraRobert A Harris
Sep 28, 2007·The New England Journal of Medicine·Timothy W Meyer, Thomas H Hostetter
Oct 3, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wei YangTianyi Wang
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·William R WikoffGary Siuzdak
Apr 16, 2009·Journal of Proteome Research·Jinfu SunChangchun Tu
Aug 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masato HoshiMitsuru Seishima
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nicholas S HeatonGlenn Randall
Mar 1, 2011·International Immunopharmacology·Hyunkeun SongDae Young Hur
Feb 10, 2012·Kidney International·Timothy W Meyer, Thomas H Hostetter
Mar 17, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masato HoshiKuniaki Saito
Dec 25, 2012·Molecular & Cellular Proteomics : MCP·Hui SunXijun Wang
Jul 12, 2013·Science Translational Medicine·Ivan Vujkovic-CvijinJoseph M McCune
Aug 24, 2013·PLoS Neglected Tropical Diseases·Liang CuiChoon Nam Ong
Jun 15, 2014·Metabolism: Clinical and Experimental·Janne M StrandLars Eide
Aug 1, 2014·Behavioural Brain Research·S M O'MahonyJ F Cryan
Dec 17, 2014·Virus Research·Wei JiCheng-Qiang He
Apr 22, 2015·Nutrients·Evelien P J G NeisSander S Rensen
Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Lining GuoC Thomas Caskey

❮ Previous
Next ❯

Citations

Aug 9, 2018·Scientific Reports·Carlos Fernando Odir Rodrigues MeloRodrigo Ramos Catharino
Nov 30, 2019·Scientific Reports·Xinzhu WangUNKNOWN EUCLIDS consortium
Dec 29, 2020·Frontiers in Molecular Biosciences·Carolina M MalgarinJohn C S Harding

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCA
metabolomics profiling

Software Mentioned

R
Excel
MATLAB
XCMS
MassHunter Workstation
DA
Masshunter
Simca

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.