Serum Metabolomics Study Based on LC-MS and Antihypertensive Effect of Uncaria on Spontaneously Hypertensive Rats

Evidence-based Complementary and Alternative Medicine : ECAM
Ana LiuJianqing Ju

Abstract

Our previous studies have shown that Uncaria has an important role in lowering blood pressure, but its intervention mechanism has not been clarified completely in the metabolic level. Therefore, in this study, a combination method of HPLC-TOF/MS-based metabolomics and multivariate statistical analyses was employed to explore the mechanism and evaluate the antihypertensive effect of Uncaria. Serum samples were analyzed and identified by HPLC-TOF/MS, while the acquired data was further processed by partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to discover the perturbed metabolites. A clear cluster among the different groups was obtained, and 7 significantly changed potential biomarkers were screened out. These biomarkers were mainly associated with lipid metabolism (dihydroceramide, ceramide, PC, LysoPC, and TXA2) and vitamin and amino acids metabolism (nicotinamide riboside, 5-HTP). The result indicated that Uncaria could decrease the blood pressure effectively, partially by regulating the above biomarkers and metabolic pathways. Analyzing and verifying the specific biomarkers, further understanding of the therapeutic mechanism and antihypertensive effec...Continue Reading

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Citations

Dec 14, 2018·Cellular and Molecular Life Sciences : CMLS·Ruth R MagayeBing H Wang
Feb 23, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Shihua ShiHao Xu
Aug 21, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Cong HanZhen-Qiang Qi
Nov 3, 2020·Frontiers in Endocrinology·Bianca C FieldPhilipp E Scherer
Dec 31, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Jiayun WuLi Huang

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Methods Mentioned

BETA
Chemical
Profiler

Software Mentioned

SIMCA
MPP
DA
Mass Profiler Professional
OPLS
SPSS
Mass Hunter Qualitative Analysis Software
Umetrics
Mass Hunter

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