Serum metabonomics study on antidiabetic effects of fenugreek flavonoids in streptozotocin-induced rats

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Wenyue JiangLu Gao

Abstract

Fenugreek is a well-known medicinal plant used for treatment of diabetes. In this study, the antidiabetic effect of fenugreek flavonoids was investigated by metabonomics based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Fenugreek flavonoids were purified using polyamide resin and D101 macroporous adsorption resin, characterized by UPLC-Q-TOF-MS, and administered to streptozotocin (STZ)-induced diabetic rats for 28 days. Pharmacological study results indicated that fenugreek flavonoids exerted a strong antidiabetic effect characterized by significant reduction of fasting blood glucose (P < 0.01), increase in serum insulin level (P < 0.01) and liver glycogen content (P < 0.01), attenuation of weight loss, and improvement of pancreatic islet and kidney conditions. The antidiabetic effect of fenugreek flavonoids was further analyzed by metabonomics. Serum samples of health and diabetic rats treated or not with fenugreek flavonoids were evaluated by UPLC-Q-TOF-MS, followed by principal component analysis (PCA) and orthogonal projection to latent structures squares-discriminant analysis (OPLS-DA). The PCA model revealed significant differences among the animal g...Continue Reading

Citations

Jan 18, 2020·Chemistry & Biodiversity·Zi-Li KongJing-Wei Chi
Aug 3, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jiasheng WuYueming Ma

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