Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells.

Molecular Medicine Reports
Yingjuan ZhengXiaoyan Xu

Abstract

The actions of thyroid hormone (TH) on lipid metabolism in the liver are associated with a number of genes involved in lipogenesis and lipid metabolism; however, the underlying mechanisms through which TH impacts on lipid metabolism remain to be elucidated. The present study aimed to investigate the effects of hyperthyroidism on the serum levels of the microRNA (miR) miR‑206 and the role of miR‑206 on TH‑regulated lipid metabolism in liver cells. Serum was obtained from 12 patients diagnosed with hyperthyroidism and 10 healthy control subjects. Human hepatoblastoma (HepG2) cells were used to study the effects of triiodothyronine (T3) and miR‑206 on lipid metabolism. Expression of miR‑206 in serum and cells was determined by reverse transcription‑quantitative polymerase chain reaction analysis. Lipid accumulation in HepG2 cells was assessed with Oil Red O staining. Suppression or overexpression of miR‑206 was performed via transfection with a miR‑206 mimic or miR‑206 inhibitor. Serum miR‑206 was significantly decreased in patients with hyperthyroidism compared with euthyroid controls. Treatment of HepG2 cells with T3 led to reduced total cholesterol (TC) and triglyceride (TG) content, accompanied by reduced miR‑206 expression. I...Continue Reading

References

Jan 1, 1991·The Journal of Clinical Investigation·J H OppenheimerM P Thompson
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 21, 2003·Lancet·David S Cooper
Jun 15, 2005·BMC Physiology·Nina StåhlbergAmilcar Flores-Morales
Oct 11, 2005·The Journal of Endocrinology·K Boelaert, J A Franklyn
Apr 11, 2009·World Journal of Gastroenterology : WJG·Xian-Ming Chen
Jul 2, 2009·The Journal of Clinical Endocrinology and Metabolism·W Edward VisserTheo J Visser
Sep 16, 2009·Human Pathology·Dolores López-TerradaBarbara B Knowles
Oct 6, 2009·Trends in Endocrinology and Metabolism : TEM·Francis C Lynn
Jan 7, 2010·Endocrine Reviews·Sheue-Yann ChengPaul J Davis
May 10, 2011·Annual Review of Nutrition·Kathryn J MooreCarlos Fernández-Hernando
Jun 12, 2012·The Journal of Clinical Investigation·Rohit Anthony SinhaPaul M Yen
Jul 11, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kasey C VickersPraveen Sethupathy
Jul 13, 2012·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Timothy J ShoemakerCarmella Evans-Molina
Sep 5, 2012·The Journal of Clinical Investigation·Gregory A Brent
Sep 6, 2012·Endocrinology·Ira J GoldbergJoel R Ehrenkranz
Nov 16, 2012·The Journal of Endocrinology·Aline CordeiroCarmen Cabanelas Pazos-Moura
Mar 19, 2013·Cellular Signalling·Dan ZhongFengtian He
Mar 19, 2013·Journal of Lipid Research·Kasey C VickersAlan T Remaley
Oct 10, 2013·Biochemical and Biophysical Research Communications·Chui Sun YapPaul Michael Yen
Mar 8, 2014·Biochimica Et Biophysica Acta·Manjula VinodGerhard M Kostner
May 2, 2014·European Thyroid Journal·K Alexander IwenGeorg Brabant
Jun 14, 2016·Journal of Agricultural and Food Chemistry·Akira ShibataKiyotaka Nakagawa

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Citations

Mar 27, 2019·Cancers·Ion CristóbalJesús García-Foncillas
Oct 24, 2019·International Journal of Molecular Sciences·Po-Shuan HuangKwang-Huei Lin

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