Serum of myeloproliferative neoplasms stimulates hematopoietic stem and progenitor cells

PloS One
Richard LubberichWolfgang Wagner

Abstract

Myeloproliferative neoplasms (MPN)-such as polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF)-are typically diseases of the elderly caused by acquired somatic mutations. However, it is largely unknown how the malignant clone interferes with normal hematopoiesis. In this study, we analyzed if serum of MPN patients comprises soluble factors that impact on hematopoietic stem and progenitor cells (HPCs). CD34+ HPCs were cultured in medium supplemented with serum samples of PV, ET, or MF patients, or healthy controls. The impact on proliferation, maintenance of immature hematopoietic surface markers, and colony forming unit (CFU) potential was systematically analyzed. In addition, we compared serum of healthy young (<25 years) and elderly donors (>50 years) to determine how normal aging impacts on the hematopoiesis-supportive function of serum. Serum from MF, PV and ET patients significantly increased proliferation as compared to controls. In addition, serum from MF and ET patients attenuated the loss of a primitive immunophenotype during in vitro culture. The CFU counts were significantly higher if HPCs were cultured with serum of MPN patients as compared to controls. Furthermore, serum of healthy young ...Continue Reading

References

May 2, 1994·Journal of Immunological Methods·A B Lyons, C R Parish
Aug 24, 2005·British Journal of Haematology·Katerina E PanteliKonstantinos L Bourantas
Dec 8, 2006·The New England Journal of Medicine·Peter J Campbell, Anthony R Green
Mar 3, 2007·Leukemia Research·Ching-Liang HoAyalew Tefferi
Aug 30, 2008·Leukemia·R Kralovics
May 13, 2009·Journal of Cellular and Molecular Medicine·Thomas WalendaWolfgang Wagner
Feb 9, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ayalew TefferiAnimesh Pardanani
Jul 20, 2011·The Journal of Experimental Medicine·Cornelis J H PronkSten Eirik W Jacobsen
Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wendy W PangIrving L Weissman
Feb 19, 2013·Cytokine & Growth Factor Reviews·Hans C Hasselbalch
Apr 16, 2013·Nature Reviews. Immunology·Hartmut GeigerM Carolina Florian
Jul 5, 2013·Blood·Tiziano BarbuiAnna Falanga
Jan 28, 2014·Experimental Hematology·Emmanuel PourcelotPascal Mossuz
May 27, 2015·The Journal of Clinical Investigation·Lukas F MagerPhilippe Krebs
Oct 23, 2015·Mediators of Inflammation·Vladan P ČokićPuri K Raj
Nov 6, 2015·Mediators of Inflammation·Sylvie HermouetBetty Gardie
Nov 30, 2016·Frontiers in Immunology·Larisa V KovtonyukHitoshi Takizawa

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
FCS
FACS
MDS

Software Mentioned

Excel
Flowjo
GraphPad Prism

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.