Serum opsonin ficolin-A enhances host-fungal interactions and modulates cytokine expression from human monocyte-derived macrophages and neutrophils following Aspergillus fumigatus challenge

Medical Microbiology and Immunology
Stefan BidulaSilke Schelenz

Abstract

Invasive aspergillosis is a devastating invasive fungal disease associated with a high mortality rate in the immunocompromised, such as leukaemia patients, transplant patients and those with HIV/AIDS. The rodent serum orthologue of human L-ficolin, ficolin-A, can bind to and opsonize Aspergillus fumigatus, the pathogen that causes invasive aspergillosis, and may participate in fungal defence. Using human monocyte-derived macrophages and neutrophils isolated from healthy donors, we investigated conidial association and fungal viability by flow cytometry and microscopy. Additionally, cytokine production was measured via cytometric bead arrays. Ficolin-A opsonization was observed to significantly enhance association of conidia, while also inhibiting hyphal growth and contributing to increased fungal killing following incubation with monocyte-derived macrophages and neutrophils. Additionally, ficolin-A opsonization was capable of manifesting a decrease in IL-8, IL-1β, IL-6, IL-10 and TNF-α production from MDM and IL-1β, IL-6 and TNF-α from neutrophils 24 h post-infection. In conclusion, rodent ficolin-A is functionally comparable to human L-ficolin and is capable of modulating the innate immune response to A. fumigatus, down-regula...Continue Reading

References

Feb 2, 1996·The Journal of Biological Chemistry·M MatsushitaT Mizuochi
Dec 12, 1997·The Journal of Infectious Diseases·M L GrazziuttiC A Savary
Jan 24, 2003·Infection and Immunity·O Ibrahim-GranetJ P Latgé
Dec 9, 2003·Nature Reviews. Immunology·Luigina Romani
Dec 3, 2005·The Journal of Infection·Zhihong ZhangHenk F Kauffman
Dec 3, 2005·Proceedings of the American Thoracic Society·Thomas R Martin, Charles W Frevert
Mar 6, 2007·Clinical and Experimental Immunology·S KaurT Madan
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Umakhanth Venkatraman GirijaRussell Wallis
May 1, 2009·The New England Journal of Medicine·Brahm H Segal
Jun 23, 2009·Blood·Matteo BianchiJanine Reichenbach
Jul 28, 2009·The Journal of Biological Chemistry·Ying Jie MaPeter Garred
Oct 16, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jonathan LambourneThomas S Harrison
Mar 20, 2010·PloS One·Brahm H SegalTimothy S Blackwell
Jun 6, 2012·PloS One·Tina HummelshøjPeter Garred
Aug 18, 2012·The Journal of Infection·William J SteinbachDavid L Horn
Sep 11, 2012·Molecular Immunology·Misao Matsushita
Jul 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ying Jie MaPeter Garred
May 1, 2015·Critical Care : the Official Journal of the Critical Care Forum·Fabio Silvio TacconeUNKNOWN AspICU Study Investigators

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Citations

May 17, 2011·International Journal of Medical Microbiology : IJMM·Mike HasenbergMatthias Gunzer
Jan 27, 2018·Journal of Fungi·Brendan D SnarrDonald C Sheppard
Jan 30, 2020·FEBS Letters·Raffaella ParenteAntonio Inforzato

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