Abstract
Invasive aspergillosis is a devastating invasive fungal disease associated with a high mortality rate in the immunocompromised, such as leukaemia patients, transplant patients and those with HIV/AIDS. The rodent serum orthologue of human L-ficolin, ficolin-A, can bind to and opsonize Aspergillus fumigatus, the pathogen that causes invasive aspergillosis, and may participate in fungal defence. Using human monocyte-derived macrophages and neutrophils isolated from healthy donors, we investigated conidial association and fungal viability by flow cytometry and microscopy. Additionally, cytokine production was measured via cytometric bead arrays. Ficolin-A opsonization was observed to significantly enhance association of conidia, while also inhibiting hyphal growth and contributing to increased fungal killing following incubation with monocyte-derived macrophages and neutrophils. Additionally, ficolin-A opsonization was capable of manifesting a decrease in IL-8, IL-1β, IL-6, IL-10 and TNF-α production from MDM and IL-1β, IL-6 and TNF-α from neutrophils 24 h post-infection. In conclusion, rodent ficolin-A is functionally comparable to human L-ficolin and is capable of modulating the innate immune response to A. fumigatus, down-regula...Continue Reading
References
Feb 2, 1996·The Journal of Biological Chemistry·M MatsushitaT Mizuochi
Aug 1, 1997·Infection and Immunity·T MadanK B Reid
Dec 12, 1997·The Journal of Infectious Diseases·M L GrazziuttiC A Savary
Jul 28, 1999·Medical Mycology·S SchelenzG J Bancroft
Aug 24, 1999·Infection and Immunity·M J AllenR J Mason
Mar 20, 2001·Infection and Immunity·T MadanP U Sarma
Jan 11, 2003·Medical Mycology·Y ArataniH Koyama
Jan 24, 2003·Infection and Immunity·O Ibrahim-GranetJ P Latgé
Oct 15, 2003·The Journal of Infection·S Schelenz, D J A Goldsmith
Dec 9, 2003·Nature Reviews. Immunology·Luigina Romani
Sep 21, 2004·Respiratory Research·Laura L KothDavid J Erle
Dec 3, 2005·The Journal of Infection·Zhihong ZhangHenk F Kauffman
Dec 3, 2005·Proceedings of the American Thoracic Society·Thomas R Martin, Charles W Frevert
Sep 7, 2006·Cellular Microbiology·Kathrin LutherFrank Ebel
Mar 6, 2007·Clinical and Experimental Immunology·S KaurT Madan
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Umakhanth Venkatraman GirijaRussell Wallis
Aug 16, 2008·The Journal of Biological Chemistry·Viviane BalloyMichel Chignard
May 1, 2009·The New England Journal of Medicine·Brahm H Segal
Jun 23, 2009·Blood·Matteo BianchiJanine Reichenbach
Jul 14, 2009·The Journal of Infectious Diseases·Monica M MircescuTobias M Hohl
Jul 28, 2009·The Journal of Biological Chemistry·Ying Jie MaPeter Garred
Sep 22, 2009·Molecular Immunology·Maciej CedzynskiDavid C Kilpatrick
Oct 16, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jonathan LambourneThomas S Harrison
Mar 20, 2010·PloS One·Brahm H SegalTimothy S Blackwell
May 6, 2010·PLoS Pathogens·Sandra BrunsMatthias Gunzer
Aug 3, 2011·Infection and Immunity·Jessica L WernerChad Steele
Nov 1, 2011·Infection and Immunity·Melissa A GessnerChad Steele
Mar 28, 2012·Infection and Immunity·Mioko FujiedaShinji Takahashi
Jun 6, 2012·PloS One·Tina HummelshøjPeter Garred
Aug 18, 2012·The Journal of Infection·William J SteinbachDavid L Horn
Sep 11, 2012·Molecular Immunology·Misao Matsushita
Dec 6, 2012·Medical Mycology·David W DenningDonald C Cole
Mar 13, 2013·Infection and Immunity·Stefan BidulaSilke Schelenz
Jul 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ying Jie MaPeter Garred
Jul 26, 2013·BMB Reports·Ying Jie MaBok Luel Lee
Sep 17, 2013·PloS One·Fengling LuoXiao-Lian Zhang
Jan 24, 2015·The Journal of Infectious Diseases·Stefan BidulaSilke Schelenz
May 1, 2015·Critical Care : the Official Journal of the Critical Care Forum·Fabio Silvio TacconeUNKNOWN AspICU Study Investigators
Citations
May 17, 2011·International Journal of Medical Microbiology : IJMM·Mike HasenbergMatthias Gunzer
Jul 16, 2016·PLoS Pathogens·Stefan Bidula, Silke Schelenz
Jan 7, 2017·Virulence·Kasper JensenGrith L Sorensen
Apr 22, 2017·Experimental & Molecular Medicine·Ying Jie MaPeter Garred
Jan 27, 2018·Journal of Fungi·Brendan D SnarrDonald C Sheppard
Mar 12, 2016·Journal of Cell Science·Xinjian PengYing Wang
Feb 28, 2018·Scientific Reports·Chunxiao LiGuangwei Liu
Jan 30, 2020·FEBS Letters·Raffaella ParenteAntonio Inforzato
Mar 15, 2019·Journal of Immunology Research·Stefan BidulaSilke Schelenz
Feb 5, 2021·Scientific Reports·Nongnuch SingrangKittikhun Wangkanont