Serum profile of cytokines interferon gamma and interleukin-10 in ewes subjected to artificial insemination by cervical retraction

Theriogenology
C T G AlvaresF Z Brandão

Abstract

This study evaluated the influence of artificial insemination (AI) by cervical retraction (CRI) on serum levels of interferon gamma (IFNγ) and interleukin-10 (IL-10) in ewes. Synchronized pluriparous Santa Inês ewes were subjected to natural mating (NM, n = 8) and AI, which was performed for a fixed time (55 ± 1 hour) by CRI (n = 8) or laparoscopy (n = 8). Ewes were classified as pregnant, with return to estrus (RE) or with embryonic loss (EL). Blood samples were collected on Day 0, Day 3, Day 5, Day 12, and Day 17 (Day 0 = AI/NM) for progesterone dosage and cytokines were quantified from Day 0 to Day 12. Progesterone levels were constant, except for a decrease in ewes with RE at Day 17 (P < 0.05). Regardless of the reproductive method used, there was no difference in the IFNγ and IL-10 levels at any time, with averages of 642.1, 713.2, and 741.2 pg/mL for IFNγ and 667.1, 616.8, and 721.1 pg/mL for IL-10 when using CRI, laproscopy, and NM, respectively. Regarding the physiological status, ewes with EL had lower serum levels of IFNγ and IL-10 than pregnant ewes and ewes with RE, regardless of the reproductive method used, with averages of 769.1, 714.9, and 555.7 pg/mL for IFNγ and 713.8, 699.3, and 578.7 pg/mL for IL-10 in pregn...Continue Reading

References

Jan 26, 1999·Cytokine & Growth Factor Reviews·A Rice, T Chard
Aug 5, 2000·Animal Reproduction Science·S Salamon, W M Maxwell
Aug 26, 2000·Cytokine & Growth Factor Reviews·S E Dorman, S M Holland
Aug 31, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·S E MitchellL M Williams
Jan 22, 2003·Animal Reproduction Science·S Schäfer-Somi
Apr 12, 2005·Fertility and Sterility·Jiae LeeJeong-Wook Kim
Jun 14, 2005·Fertility and Sterility·Elizabeth S GinsburgJoseph A Hill
Feb 24, 2006·The Veterinary Journal·C W WeemsR D Randel
May 10, 2006·Seminars in Fetal & Neonatal Medicine·Ole B ChristiansenAstrid M Kolte
May 1, 1990·Theriogenology·G W HalbertB C Buckrell
Jun 9, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Fuller W BazerGuoyao Wu
Nov 6, 2009·Journal of Reproductive Immunology·Yi LinShigeru Saito
Feb 22, 2011·Journal of Reproductive Immunology·Gendie E Lash, Judith N Bulmer
Feb 22, 2011·Journal of Reproductive Immunology·Satyan KalkunteSurendra Sharma
Jun 21, 2011·Journal of Reproductive Immunology·Antonis MakrigiannakisUdo Jeschke
Aug 30, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Deng-Xuan FanLi-Ping Jin

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Citations

Oct 19, 2019·Reproduction in Domestic Animals = Zuchthygiene·Mercedes AlvarezLuis Anel

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