Serum profiling of leptospirosis patients to investigate proteomic alterations.

Journal of Proteomics
Rajneesh SrivastavaSanjeeva Srivastava

Abstract

Leptospirosis is a zoonotic infectious disease of tropical, subtropical and temperate zones, which is caused by the pathogenic spirochetes of genus Leptospira. Although this zoonosis is generally not considered as fatal, the pathogen can eventually cause severe infection with septic shock, multi-organ failure and lethal pulmonary hemorrhages leading to mortality. In this study, we have performed a proteomic analysis of serum samples from leptospirosis patients (n=6), febrile controls (falciparum malaria) (n=8) and healthy subjects (n=18) to obtain an insight about disease pathogenesis and host immune responses in leptospiral infections. 2DE and 2D-DIGE analysis in combination with MALDI-TOF/TOF MS revealed differential expression of 22 serum proteins in leptospirosis patients compared to the healthy controls. Among the identified differentially expressed proteins, 8 candidates exhibited different trends compared to the febrile controls. Functional analysis suggested the involvement of differentially expressed proteins in vital physiological pathways, including acute phase response, complement and coagulation cascades and hemostasis. This is the first report of analysis of human serum proteome alterations in leptospirosis patien...Continue Reading

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Citations

Aug 16, 2013·International Journal of Nanomedicine·Chien-Ling SuUNKNOWN Taiwan CardioPulmonary Research Group (T-CPR)
Dec 3, 2013·Journal of Hazardous Materials·Tzu-Tao ChenTsun-Jen Cheng
Oct 20, 2012·Journal of Proteomics·Lello Zolla, Angelo D'Alessandro
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Mar 7, 2020·Critical Reviews in Microbiology·Monica L VieiraAna Lucia T O Nascimento

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Methods Mentioned

BETA
electrophoresis
Protein Assay
proteomic profiling

Software Mentioned

DAVID
Image Master 2D Platinum
[UNK] Explorer
LabScan
Biocarta
IMP7
PANTHER
ImageMaster 2D Platinum
2DE
MASCOT

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