Serum Protein Adsorption Enhances Active Leukemia Stem Cell Targeting of Mesoporous Silica Nanoparticles

ACS Applied Materials & Interfaces
Michaela BeckMika Lindén

Abstract

The functionalization of nanoparticles with a ligand targeting receptors overexpressed by the target cells is a commonly used strategy when aiming at nanoparticle-based, cell type-specific drug delivery.1-4 However, the influence of particle surface chemistry on the targetability has received much less attention. The surface charge is known to directly or indirectly affect the nanoparticle cellular uptake kinetics by influencing serum protein adsorption.5-7 Thus, it is fair to assume that both the specificity and cellular uptake kinetics of targeted nanoparticles are influenced by the nanoparticle charge, both of which are important parameters for controlling cell-specific drug delivery efficiency. We therefore studied the influence of the surface chemistry of mesoporous silica nanoparticles (MSNs) carrying identical amounts of a specific antibody (anti-B220) on the selectivity toward B220-positive leukemia stem cells. The uptake by these cells was higher compared to the nanoparticle uptake by B220-negative leukemia stem cells, demonstrating uptake specificity. In addition, the adsorption of serum proteins onto the differently charged MSNs was studied by SDS-PAGE. Interestingly, the highest selectivity was not observed for the ...Continue Reading

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Citations

Mar 7, 2019·Advanced Healthcare Materials·Haiwei WuXuejian Liu
Mar 23, 2019·Expert Opinion on Drug Delivery·Rafael R CastilloMaría Vallet-Regí
Jul 13, 2019·Nanomedicine·Feby Wijaya PratiwiPeilin Chen
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Hainan SunShumei Zhai
Jan 24, 2021·Journal of Colloid and Interface Science·Cornelia von BaeckmannFreddy Kleitz
Jan 19, 2021·Chemical Research in Toxicology·Ozge KoseValérie Forest

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