Nov 13, 2019

Serum Protein Changes in Pediatric Sepsis Patients Identified With an Aptamer-Based Multiplexed Proteomic Approach

Critical Care Medicine
Nicholas J ShubinAdrian M Piliponsky

Abstract

Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response to infection, is a leading cause of death and disability among children worldwide. Identifying sepsis in pediatric patients is difficult and can lead to treatment delay. Our objective was to assess the host proteomic response to infection utilizing an aptamer-based multiplexed proteomics approach to identify novel serum protein changes that might help distinguish between pediatric sepsis and infection-negative systemic inflammation and hence can potentially improve sensitivity and specificity of the diagnosis of sepsis over current clinical criteria approaches. Retrospective, observational cohort study. PICU and cardiac ICU, Seattle Children's Hospital, Seattle, WA. A cohort of 40 children with clinically overt sepsis and 30 children immediately postcardiopulmonary bypass surgery (infection-negative systemic inflammation control subjects) was recruited. Children with sepsis had a confirmed or suspected infection, two or more systemic inflammatory response syndrome criteria, and at least cardiovascular and/or pulmonary organ dysfunction. None. Serum samples from 35 of the sepsis and 28 of the bypass surgery subjects were available for screening w...Continue Reading

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Mentioned in this Paper

Study
Hospitals, Pediatric
Pediatric Intensive Care Unit
Genes
Trees (plant)
Bypass Surgery
Cessation of Life
Intensive Care Unit
Proteome
Lung Diseases

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