Serum small extracellular vesicle-derived LINC00853 as a novel diagnostic marker for early hepatocellular carcinoma.

Molecular Oncology
Soon Sun KimJung Woo Eun

Abstract

This study aimed to identify novel long noncoding RNA (lncRNA) biomarkers for hepatocellular carcinoma (HCC) using publicly available tissue genomic datasets and validate their diagnostic utility for early-stage HCC. Differentially expressed lncRNAs between 371 HCC and 50 nontumor tissues were obtained from The Cancer Genome Atlas liver hepatocellular carcinoma (TCGA_LIHC) project. Subsequently, the expression of the serum- and extracellular vesicle (EV)-derived lncRNA was assessed in 10 patients with HCC and 10 healthy controls using RT-qPCR. The candidate lncRNAs were validated in 90 HCC and 92 non-HCC (29 healthy control, 28 chronic hepatitis, 35 liver cirrhosis) patients. The sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) were calculated for the candidate lncRNAs and the current HCC biomarker, alpha-fetoprotein (AFP). SFTA1P, HOTTIP, HAGLROS, LINC01419, HAGLR, CRNDE, and LINC00853 were markedly upregulated in HCC in TCGA_LIHC dataset. Among them, LINC00853 has not been reported in relation to HCC before. In patients with HCC, only expression of small EV-derived LINC00853 (EV-LINC00853) was increased. EV-LINC00853 showed excellent discriminatory ability in the diagnosis of all-stag...Continue Reading

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Citations

Sep 16, 2020·Seminars in Cancer Biology·Pengbo CaoGangqiao Zhou
Apr 4, 2021·International Journal of Molecular Sciences·Lok-Sze Wong, Chun-Ming Wong
Apr 24, 2021·World Journal of Gastrointestinal Oncology·Ming-Cheng GuanTian Yang
May 28, 2021·World Journal of Gastrointestinal Oncology·Ming-Cheng GuanTian Yang
Aug 1, 2021·Pathology, Research and Practice·Chuyi ZengHualin Tao
Oct 29, 2021·Cancer Medicine·Natthaphong NimitrungtaweeNipon Chattipakorn

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Datasets Mentioned

BETA
GSE124535
GSE114564

Methods Mentioned

BETA
reverse transcription PCR
biopsy
serum collection

Software Mentioned

graphpad prism
nta
SPSS
GraphPad

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