Serum stimulation of phosphate uptake into 3T3 cells

Journal of Cellular Physiology
D A Hilborn

Abstract

The stimulation by calf serum of phosphate uptake into 3T3 cells results from a change in maximum velocity of the transport process with no change in the Michaelis constant. Only arsenate among a series of inorganic structural analogs of phosphate inhibited phosphate uptake indicating a high specificity for the process. The arsenate inhibition was competitive in nature. Papaverine, theophylline, and protaglandin E1, drugs known to maintain high intracellular levels of cAMP, had little effect on serum stimulated phosphate uptake. The phosphate uptake stimulating factor(s) in serum could be distinguised from the 3T3 cell survival and migration factors by stability characteristics, but this factor(s) could not be completely separated from a uridine uptake stimulation activity or growth promoting activity using a variety of serum fractionation procedures. Only partial stimulation of the uptake process was achieved with any one serum fraction indicating a multiplicity of serum components is probably involved in this process. Because of the rapidity of serum activation of phosphate uptake and its apparent independence of intracellular cyclic nucleotide levels, it is suggested that serum factors may stimulate phosphate uptake by induc...Continue Reading

References

Nov 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D D Cunningham, A B Pardee
Oct 1, 1972·Experimental Cell Research·A LiptonR W Holley
Oct 11, 1972·Nature: New Biology·M M BurgerJ R Sheppard
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E Rozengurt, L Jimenez de Asua
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L J de AsuaR Dulbecco
Feb 27, 1974·Biochemical and Biophysical Research Communications·P G Plagemann, J R Sheppard
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R W Holley
Oct 1, 1971·Journal of Cellular Physiology·H M Temin
Mar 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D PaulI Klinger
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A LiptonR W Holley
Apr 1, 1971·The Journal of Cell Biology·J D ChapmanJ Borsa

Citations

Jan 1, 1979·Bulletin of Environmental Contamination and Toxicology·E J Brown, D K Button
Jul 9, 1999·Progress in Lipid Research·C M Lermer, R D Mattes
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M J Warburton, B Poole
Feb 11, 2010·Cancer Prevention Research·Corinne E CamalierGeorge R Beck
Aug 1, 1981·Environmental Health Perspectives·K W Jennette
Jan 3, 2013·Journal of Cellular Physiology·Corinne E CamalierGeorge R Beck
Jan 1, 1980·Annals of the New York Academy of Sciences·J E Lever
Nov 1, 1979·Journal of Cellular Physiology·M L Smith, J M Buchanan
Mar 1, 1979·Journal of Cellular Physiology·W R AllenD Gospodarowicz
Nov 1, 1977·Journal of Cellular Physiology·D C Quinlan, J Hochstadt
Dec 14, 1979·Molecular and Cellular Biochemistry·A B Stavitsky, A A Gerblich
Jan 1, 1977·Journal of Supramolecular Structure·G S Barsh, D D Cunningham

Related Concepts

Cyclic AMP, (R)-Isomer
Adenosine Triphosphate, Chromium Ammonium Salt
Arsenates
Blood
Plasma Membrane
Chromium
Hydrogen-Ion Concentration
Novolin
Pavatym
Orthophosphate

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