Serum thymic factor prevents LPS-induced pancreatic cell damage in mice via up-regulation of Bcl-2 expression in pancreas

Microbiology and Immunology
Noriko SaitohTakashi Onodera

Abstract

The protective effect of synthetic serum thymic factor (FTS) nonapeptide on lipopolysaccharide (LPS)-induced pancreatic cell damage in 10-week-old BALB/c male mice was investigated. Mice were divided into three groups. Group I was treated with LPS (10 micro g/head; i.p.) (LPS-treated mice). Group II was administered with FTS (50 micro g/head; i.p.) 24 hr before treatment with LPS and complemented immediately before LPS injection with FTS (50 micro g/head; i.p.) (FTS-administered mice). Group III was only treated with the same volume of saline (control mice). Treatment of LPS in vivo resulted in the destruction of pancreatic acinar cells. In those cells, many apoptotic cells were detected by immunohistochemistry using an anti-single stranded DNA antibody. Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) revealed that LPS treatment also caused low or a lack of insulin expression in pancreatic islets. In contrast, morphological change was not seen and apoptotic cell death was suppressed in pancreatic cells of FTS-administered mice. Moreover, insulin expression was normal in those mice. FTS administration enhanced expression of Bcl-2 mRNA levels in pancreatic tissues and IL-6 mRNA levels in splenocy...Continue Reading

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Citations

Feb 23, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Leoni Villano BonaminMario Mariano
Aug 3, 2010·Veterinary Immunology and Immunopathology·Marco FranchiniMathias Ackermann
Mar 6, 2009·Veterinary Immunology and Immunopathology·Paolo BorghettiPaolo Martelli
Feb 4, 2009·Cell Metabolism·Fabienne T SchulthessKathrin Maedler
Jun 12, 2010·Expert Opinion on Therapeutic Targets·Sergey M Lunin, Elena G Novoselova
Nov 26, 2020·International Journal of Molecular Sciences·Rita RezzaniLuigi Fabrizio Rodella

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