PMID: 7537870Apr 11, 1995Paper

Seryl-tRNA synthetase from Escherichia coli: functional evidence for cross-dimer tRNA binding during aminoacylation

Nucleic Acids Research
C VincentM Härtlein

Abstract

Escherichia coli seryl-tRNA synthetase (SerRS) is a homo-dimeric class II aminoacyl-tRNA synthetase. Each subunit is composed of two distinct domains: the N-terminal domain is a 60 A long, arm-like coiled coil structure built up of two antiparallel alpha-helices, whereas the C-terminal domain, the catalytic core, is an alpha-beta structure overlying a seven-stranded antiparallel beta-sheet. Deletion of the arm-like domain (SerRS delta 35-97) does not affect the amino acid activation step of the reaction, but reduces aminoacylation activity by more than three orders of magnitude. In the present study, it was shown that the formation of heterodimers from two aminoacylation defective homodimers, the N-terminal deletion and an active site mutant (SerRS E355Q), restored charging activity. The aminoacylation activity in a mixture containing the heterodimers was compared to that of solutions containing the same concentrations of homodimer. The activity of the mixture was eight times higher than the activities of the homodimer solutions, and reached 50% of the theoretical value that would be expected if 50% of the mixture was in the heterodimer form and assuming that a heterodimer contains only one active site. These results are in ful...Continue Reading

References

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Citations

Jan 21, 2004·Biochimica Et Biophysica Acta·Jitsuhiro Matsugi, Katsutoshi Murao
Jan 22, 1998·Current Opinion in Structural Biology·S Cusack
Oct 1, 1995·Nature Structural Biology·S Cusack
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rita RochaSandra Macedo-Ribeiro
Feb 21, 2009·The Journal of Biological Chemistry·Silvija BilokapicIvana Weygand-Durasevic
Jan 16, 1999·Nucleic Acids Research·B LenhardI Weygand-Durasević
Nov 4, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jean Baptiste ArteroMichael Haertlein
Jul 11, 2006·Journal of Molecular Biology·Ita Gruic-SovuljIvana Weygand-Durasevic
Mar 21, 2015·The Journal of Physical Chemistry. B·Saheb Dutta, Nilashis Nandi
Oct 1, 2011·Journal of Zhejiang University. Science. B·Ya-nan ZhangLi-jun Zhong
Aug 11, 2016·Nucleic Acids Research·Irem Avcilar-KucukgozeZoya Ignatova
Jun 27, 2019·Journal of Biomolecular Structure & Dynamics·Amrita SahaNilashis Nandi

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