Sesbania mosaic virus (SeMV) infectious clone: possible mechanism of 3' and 5' end repair and role of polyprotein processing in viral replication.

PloS One
Kunduri GovindH S Savithri

Abstract

Sesbania mosaic virus (SeMV) is a positive stranded RNA virus belonging to the genus Sobemovirus. Construction of an infectious clone is an essential step for deciphering the virus gene functions in vivo. Using Agrobacterium based transient expression system we show that SeMV icDNA is infectious on Sesbania grandiflora and Cyamopsis tetragonoloba plants. The efficiency of icDNA infection was found to be significantly high on Cyamopsis plants when compared to that on Sesbania grandiflora. The coat protein could be detected within 6 days post infiltration in the infiltrated leaves. Different species of viral RNA (double stranded and single stranded genomic and subgenomic RNA) could be detected upon northern analysis, suggesting that complete replication had taken place. Based on the analysis of the sequences at the genomic termini of progeny RNA from SeMV icDNA infiltrated leaves and those of its 3' and 5' terminal deletion mutants, we propose a possible mechanism for 3' and 5' end repair in vivo. Mutation of the cleavage sites in the polyproteins encoded by ORF 2 resulted in complete loss of infection by the icDNA, suggesting the importance of correct polyprotein processing at all the four cleavage sites for viral replication. C...Continue Reading

References

Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T A Gill, A T Paulson
Jun 23, 2000·Journal of Virology·T Tamm, E Truve
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Olivier VoinnetDavid Baulcombe
Apr 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sylvestre MarillonnetYuri Gleba
Jun 10, 2005·The Journal of Biological Chemistry·Panayampalli Subbian SatheshkumarHandanahal Subbarao Savithri
Nov 23, 2006·Archives of Virology·M Meier, E Truve
Jan 16, 2007·Cellular Microbiology·Vitaly CitovskyTzvi Tzfira
Feb 6, 2008·Plant Physiology·Lan-Ying Lee, Stanton B Gelvin
Mar 29, 2008·Methods in Molecular Biology·Sean N Chapman
Mar 29, 2008·Methods in Molecular Biology·Eugene V Ryabov
Jul 25, 2009·Plant Biotechnology Journal·Frank SainsburyGeorge P Lomonossoff
Sep 29, 2009·Biochimica Et Biophysica Acta·Ying LiuAniko V Paul
Oct 29, 2009·Virology·Smita Nair, H S Savithri
Nov 7, 2009·The Plant Journal : for Cell and Molecular Biology·Séverine LacombeChristophe Brugidou
Mar 26, 2010·The Journal of General Virology·John N Barr, Rachel Fearns
Nov 12, 2010·The Journal of General Virology·Allan OlspertErkki Truve
Dec 3, 2010·The FEBS Journal·Soumya Roy Chowdhury, Handanahal Subbarao Savithri
Aug 23, 2011·FEBS Letters·Allan OlspertErkki Truve

❮ Previous
Next ❯

Citations

Feb 26, 2016·Scientific Reports·Ambily AbrahamHandanahal S Savithri
Feb 17, 2017·Archives of Virology·Merike Sõmera, Erkki Truve
Jan 24, 2015·Archives of Virology·Merike Sõmera, Erkki Truve
Jun 6, 2018·Frontiers in Plant Science·Bernardo RodamilansJuan Antonio García
Nov 16, 2018·Archives of Virology·G P Vishnu VardhanM R N Murthy
Dec 25, 2021·The Journal of General Virology·Merike Sõmera Ictv Report Consortium

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY004291
FN398079.1

Methods Mentioned

BETA
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.