Sesquiterpenoids from Chloranthus anhuiensis with Neuroprotective Effects in PC12 Cells

Journal of Natural Products
Jian XuWei Qu

Abstract

Glutamate-induced excitotoxicity plays a vital role in neurodegenerative diseases. Neuroprotection against excitotoxicity has been considered as an effective experimental approach for preventing and/or treating excitotoxicity-mediated diseases. In the present study, six new sesquiterpenoids (1-6) and 26 known compounds of this type (7-32) were isolated and characterized from the whole plants of Chloranthus anhuiensis. Chlorantolide A (1) is the first example of a 5,6- seco-germacrane-type sesquiterpenoid, while phacadinane E (2) is a rare 4,5- seco-cadinane-type sesquiterpenoid. The structures of the new compounds were determined by spectroscopic analysis and by calculations of electronic circular dichroism (ECD) spectra. Their neuroprotective effects in mediating glutamate-induced PC12 cell apoptosis were evaluated. Compound 26 exhibited potent neuroprotective activity with an EC50 value of 3.3 ± 0.9 μM. Using Hoechst 33258 staining, a caspase-3 activity assay, and Western blot analysis it was demonstrated that this compound reduces the apoptosis of PC12 cells through inhibition of caspase-3 activity, while activating the Akt signaling pathway.

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Citations

Jul 19, 2018·Chemistry & Biodiversity·Hui-Lin ZhuJian Xu
Sep 6, 2020·Acta Crystallographica. Section C, Structural Chemistry·Luz ArancibiaFlorencia Di Salvo
Nov 19, 2019·Journal of Asian Natural Products Research·Yan Fei Liu, Shi-Shan Yu
Dec 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katrin KuckJörg Heilmann
Apr 27, 2021·Natural Product Research·Xu LiangJingquan Yuan

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