Sestrin 2 attenuates neonatal rat cardiomyocyte hypertrophy induced by phenylephrine via inhibiting ERK1/2

Molecular and Cellular Biochemistry
Bin DongChen Liu

Abstract

Cardiac hypertrophy is an adaptive response triggered by many physiological and pathological conditions and will lead to heart failure eventually. Sestrin 2, which is a stress-responsive protein, was reported to protect heart from ischemia reperfusion injury. However, the role of Sestrin 2 in cardiac hypertrophy remains unknown. In our present study, we aimed to explore the effects of Sestrin 2 on cardiomyocyte hypertrophy. We found that knockdown of Sestrin 2 protein aggravated cardiomyocyte hypertrophy induced by phenylephrine (PE), featured by increased hypertrophic marker ANP and cell surface area. During this process, ERK1/2 cascade was further activated, while p38, JNK1/2, and mTOR signaling pathways were not affected by downregulation of Sestrin 2. Moreover, overexpression of Sestrin 2 protein protected cardiomyocytes from PE-induced hypertrophy and ERK1/2 cascade was suppressed correspondingly. Importantly, pharmacological inhibition of ERK1/2 eliminated the exacerbated hypertrophic phenotype due to Sestrin 2 protein knockdown. In conclusion, we discovered that Sestrin 2 protected against cardiomyocyte hypertrophy induced by PE via inhibiting ERK1/2 signaling.

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Citations

Sep 7, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Yongtao ZhangHaipeng Guo
Jul 7, 2020·Disease Markers·Cao FangYingzhong Lin
Dec 24, 2019·Frontiers in Immunology·Li-Xue WangYong-Ming Yao
Dec 8, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jie Wang BJunlian Gu
Dec 15, 2020·Free Radical Biology & Medicine·Yunxia LiuNanhu Quan
Jan 12, 2021·Frontiers in Cell and Developmental Biology·Seung-Hyun RoEdward N Harris
Feb 4, 2021·International Journal of Molecular Sciences·Yoshimi KishimotoYukihiko Momiyama
Jun 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Anbo GaoLinxi Chen
Jul 7, 2021·Redox Report : Communications in Free Radical Research·Xiaojing CheWeimin Yang

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Methods Mentioned

BETA
PCR
protein assay
X-ray
transfection

Software Mentioned

Image
SPSS13
Quantity One
Pro Plus

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