Sestrin 2 is not a reductase for cysteine sulfinic acid of peroxiredoxins

Antioxidants & Redox Signaling
Hyun Ae WooSue Goo Rhee

Abstract

The active-site cysteine of 2-Cys peroxiredoxins (Prxs), a subgroup of the Prx family, is reversibly hyperoxidized to cysteine sulfinic acid during catalysis with concomitant loss of peroxidase activity. The reduction of sulfinic 2-Cys Prx enzymes, the first known biologic of such a reaction, has been reported to be catalyzed by either sulfiredoxin (Srx) or sestrin (Sesn) 2. The 13-kDa Srx and 60-kDa Sesn 2 show no sequence similarity, however. Whereas the reductase function of Srx has been confirmed by several studies, such is not the case for Sesn 2. We have now shown that (a) recombinant Sesn 2 did not catalyze the reduction of sulfinic Prx I in vitro, whereas Srx did; (b) overexpression of Sesn 2 in HeLa or A549 cells did not affect the reduction of 2-Cys Prxs, whereas overexpression of Srx markedly increased the reduction rate; and (c) the rate of sulfinic 2-Cys Prx reduction in embryonic fibroblasts derived from Sesn 2-knockout mice did not differ from that in those derived from wild-type mice. These results suggest that, unlike Srx, Sesn 2 is not a sulfinic Prx reductase.

References

Jan 25, 2002·IUBMB Life·S G RheeK Kim
May 30, 2002·Biological Chemistry·Birgit HofmannLeopold Flohé
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Dec 14, 2004·The Journal of Biological Chemistry·Hyun Ae WooSue Goo Rhee

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Citations

Sep 30, 2010·EMBO Molecular Medicine·Andrei V BudanovMichael Karin
Mar 28, 2009·Molecules and Cells·Francesc X SorianoGiles E Hardingham
Apr 17, 2009·Biochemistry·Caroline Kumsta, Ursula Jakob
Sep 16, 2010·Nature Reviews. Neuroscience·Giles E Hardingham, Hilmar Bading
Jul 16, 2010·Antioxidants & Redox Signaling·Peter L J de KeizerTobias B Dansen
Nov 19, 2010·Antioxidants & Redox Signaling·Anne-Gaëlle PlansonMichel B Toledano
Aug 18, 2010·Antioxidants & Redox Signaling·W Todd Lowther, Alexina C Haynes
Sep 28, 2010·Antioxidants & Redox Signaling·Karen F S Bell, Giles E Hardingham
Oct 23, 2010·Antioxidants & Redox Signaling·Maike ThamsenUrsula Jakob
Aug 8, 2012·Antioxidants & Redox Signaling·Joseph R BurgoynePhilip Eaton
Sep 19, 2012·Genes & Development·Thomas NyströmMikael Molin
May 9, 2013·Toxicology and Applied Pharmacology·So Hee JinSung Hwan Ki
May 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Seung-Hyun RoJun Hee Lee
Apr 2, 2011·Free Radical Research·Consuelo BorrásJose Viña
Sep 8, 2010·Free Radical Research·Isabella Dalle-DonneAldo Milzani
May 7, 2015·Expert Opinion on Therapeutic Targets·Xiaocheng Charlie Dong
Nov 21, 2015·Science·Robert A SaxtonDavid M Sabatini
May 29, 2012·Free Radical Biology & Medicine·Woojin JeongSue Goo Rhee
Mar 18, 2009·Biochimica Et Biophysica Acta·Regina Brigelius-Flohé, Anna Kipp
Jul 3, 2015·Biological & Pharmaceutical Bulletin·Kyu Min KimSung Hwan Ki
Aug 29, 2009·The EMBO Journal·Karim BensaadKaren H Vousden
Jan 30, 2013·Journal of Cellular Physiology·Fabian Sanchis-Gomar
Dec 23, 2011·The Biochemical Journal·Jisun LeeJianhua Zhang
Aug 11, 2015·Frontiers in Endocrinology·Seung-Hyun RoJun Hee Lee
Apr 7, 2011·Free Radical Biology & Medicine·Kahina AbbasJean-Claude Drapier
Jul 4, 2012·Free Radical Biology & Medicine·Bo Yeon ShinSung Hwan Ki
Sep 24, 2013·Cell Metabolism·Jun Hee LeeMichael Karin
May 5, 2011·Biochimica Et Biophysica Acta·David E A Kloet, Boudewijn M T Burgering
Mar 21, 2015·International Journal of Endocrinology·Dandan ChaiZhiwen Yu
May 14, 2016·Trends in Biochemical Sciences·Allison HoJun Hee Lee
May 18, 2016·Cells·Laura E GallagherEdmond Y W Chan
Jul 14, 2016·Médecine sciences : M/S·Fanny LebourgMichel Lepoivre
Aug 2, 2017·Antioxidants & Redox Signaling·Elizabeth A VealChé S Pillay
Nov 30, 2013·Nature Reviews. Drug Discovery·Chiara GorriniTak W Mak

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