Sestrins are differentially expressed with age in the skeletal muscle of men: A cross-sectional analysis

Experimental Gerontology
Nina ZengDavid Cameron-Smith

Abstract

A gradual loss of skeletal muscle mass is a common feature of aging, leading to impaired insulin sensitivity and mobility. Sestrin1, 2, 3 are multifunctional proteins that regulate the mammalian target of rapamycin complex (mTORC1), autophagy and redox homeostasis. It is unclear how aging affects Sestrins and their downstream targets in human, therefore this study examined the basal expression of Sestrins in three age groups, young, middle-aged and older men and explored the mTORC1 pathway, autophagy markers and antioxidant regulation. Older men had less Sestrin1 and 3 protein and a different pattern of Sestrin2 electrophoretic mobility. The mRNA expression of SESN1 was upregulated in older men, but the discrepancy was not by microRNA expression. Although protein expressions of Sestrins were downregulated with aging, phosphorylation of AMP-dependent protein kinase (AMPKαThr172) and read-outs of mTORC1 activation, ribosomal protein S6 kinase 1 (p70S6K1Thr421/Ser424) and 4E-binding protein 1 (4E-BP1) mobility shift were unaltered. However, total p70S6K1 and 4E-BP1 were reduced in middle-aged and older men. The mRNA expressions of autophagic markers including microtubule-associated protein 1 light chain 3 (LC3) and BCL2 interactin...Continue Reading

Citations

Sep 24, 2020·The New England Journal of Medicine·J A Jeevendra Martyn, Masao Kaneki
Jun 18, 2020·Frontiers in Medicine·Katsunori YanaiYoshiyuki Morishita
Jun 18, 2019·Oxidative Medicine and Cellular Longevity·Marco CordaniMassimo Donadelli
Aug 26, 2018·Biogerontology·Guillermo López-LluchPlácido Navas
Jan 14, 2020·Nature Communications·Jessica SegalésPura Muñoz-Cánoves
Feb 23, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Omeed RahimiPatricia E Gallagher
Oct 7, 2020·Mechanisms of Ageing and Development·Alexander Haidurov, Andrei V Budanov
Oct 29, 2020·Annual Review of Physiology·Myungjin KimJun Hee Lee
Dec 29, 2020·Frontiers in Cell and Developmental Biology·Cristiana PerrottaClara De Palma
Jan 12, 2021·Frontiers in Cell and Developmental Biology·Seung-Hyun RoEdward N Harris
Jan 21, 2021·American Journal of Physiology. Cell Physiology·Nina ZengCameron J Mitchell

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