Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3

The Journal of Biological Chemistry
M TachibanaY Shinkai

Abstract

The covalent modification of histone tails has regulatory roles in various nuclear processes, such as control of transcription and mitotic chromosome condensation. Among the different groups of enzymes known to catalyze the covalent modification, the most recent additions are the histone methyltransferases (HMTases), whose functions are now being characterized. Here we show that a SET domain-containing protein, G9a, is a novel mammalian lysine-preferring HMTase. Like Suv39 h1, the first identified lysine-preferring mammalian HMTase, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10-20-fold higher activity. It was reported that lysines 4, 9, and 27 in H3 are methylated in mammalian cells. G9a was able to add methyl groups to lysine 27 as well as 9 in H3, compared with Suv39 h1, which was only able to methylate lysine 9. Our data clearly demonstrated that G9a has an enzymatic nature distinct from Suv39 h1 and its homologue h2. Finally, fluorescent protein-labeled G9a was shown to be localized in the nucleus but not in the repressive chromatin domains of centromeric loci, in which Suv39 h1 family proteins were localized. This finding indicates that G9a may contribute to the organization of the higher ...Continue Reading

References

Jan 1, 1989·Methods in Enzymology·C von HoltB T Sewell
Jan 1, 1997·Trends in Genetics : TIG·S Smith, T de Lange
Feb 26, 1998·Cell·B van SteenselT de Lange
Mar 6, 1998·Cellular and Molecular Life Sciences : CMLS·T JenuweinG Reuter
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y WeiC D Allis
Jun 26, 1999·Science·D ChenM R Stallcup
Aug 10, 1999·Seminars in Cell & Developmental Biology·S ThomsonA L Clayton
Oct 3, 1999·Nature Biotechnology·M V MatzS A Lukyanov
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·B D StrahlC D Allis
Jan 19, 2000·Nature·B D Strahl, C D Allis

❮ Previous
Next ❯

Citations

Oct 24, 2002·Genesis : the Journal of Genetics and Development·Nobuhiro ShimozawaMamoru Ito
Feb 5, 2010·Journal of Mammary Gland Biology and Neoplasia·Mathieu Dalvai, Kerstin Bystricky
Feb 4, 2011·Journal of Biosciences·Dwaipayan Adhya, Anirban Basu
Mar 17, 2004·Biochimica Et Biophysica Acta·H Karimi Kinyamu, Trevor K Archer
Oct 31, 2003·Trends in Genetics : TIG·Robert J SimsDanny Reinberg
Apr 23, 2004·Trends in Plant Science·Peter Loidl
Jun 5, 2003·Archives of Biochemistry and Biophysics·Robert L Houtz, Archie R Portis
Feb 5, 2003·Biochemical and Biophysical Research Communications·Ken Yamamoto, Miki Sonoda
Jul 20, 2002·Trends in Genetics : TIG·Neil Brockdorff
Jun 5, 2003·Medical Hypotheses·Q Kong
Jun 1, 2002·Gene·Raul Alvarez-Venegas, Zoya Avramova
Jun 18, 2002·Current Opinion in Cell Biology·Monika Lachner, Thomas Jenuwein
Mar 15, 2002·Current Opinion in Genetics & Development·Tony Kouzarides
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Robert Schneider, Andrew J Bannister
Aug 2, 2003·Methods : a Companion to Methods in Enzymology·Kenichi Nishioka, Danny Reinberg
Jun 10, 2010·Cell Research·Ziqi ZhuCharlie Degui Chen
Feb 16, 2012·The Journal of Antibiotics·Masanari TakahashiMinoru Yoshida
Jul 6, 2004·Nature Cell Biology·Ryuji HamamotoYusuke Nakamura
Jan 18, 2006·Nature Cell Biology·Nirit FeldmanYehudit Bergman
Apr 29, 2008·Nature Chemical Biology·Philipp RathertAlbert Jeltsch
Feb 27, 2004·Nature Immunology·Ildikó GyoryKenneth L Wright
May 20, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jeremy J Day, J David Sweatt
Jun 19, 2007·Nature Reviews. Genetics·Lezanne Ooi, Ian C Wood
Oct 28, 2008·Nature Structural & Molecular Biology·Silvina Epsztejn-LitmanYehudit Bergman
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chandra-Prakash ChaturvediMarjorie Brand
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tanya M Spektor, Judd C Rice
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lin GuoXiaoyu Zhang
Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nilkantha Sen, Solomon H Snyder
Nov 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Danielle BittencourtMichael R Stallcup
Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chandra-Prakash ChaturvediMarjorie Brand

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.