SETD7 Regulates the Differentiation of Human Embryonic Stem Cells

PloS One
Julio CastañoMaria J Barrero

Abstract

The successful use of specialized cells in regenerative medicine requires an optimization in the differentiation protocols that are currently used. Understanding the molecular events that take place during the differentiation of human pluripotent cells is essential for the improvement of these protocols and the generation of high quality differentiated cells. In an effort to understand the molecular mechanisms that govern differentiation we identify the methyltransferase SETD7 as highly induced during the differentiation of human embryonic stem cells and differentially expressed between induced pluripotent cells and somatic cells. Knock-down of SETD7 causes differentiation defects in human embryonic stem cell including delay in both the silencing of pluripotency-related genes and the induction of differentiation genes. We show that SETD7 methylates linker histone H1 in vitro causing conformational changes in H1. These effects correlate with a decrease in the recruitment of H1 to the pluripotency genes OCT4 and NANOG during differentiation in the SETD7 knock down that might affect the proper silencing of these genes during differentiation.

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Citations

Nov 2, 2016·Nucleic Acids Research·Natasha K TuanoAssam El-Osta
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Jul 18, 2018·Signal Transduction and Targeted Therapy·Inês de Albuquerque Almeida Batista, Luisa Alejandra Helguero

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Datasets Mentioned

BETA
GSE24768

Methods Mentioned

BETA
PCR
chips
RNA-seq
Assay
immunoprecipitation
ChIP
immunoprecipitations
Infrared
acetylation
co-immunoprecipitation

Software Mentioned

ENCODE
DAVID
Affymetrix GCOS
GSEAPreranked
GRAMS
R
GENE

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