PMID: 7539145May 23, 1995Paper

Sets of EcoRI fragments containing ribosomal RNA sequences are conserved among different strains of Listeria monocytogenes

Proceedings of the National Academy of Sciences of the United States of America
J L BruceJ A Webster

Abstract

To classify Listeria monocytogenes using taxonomic characters derived from the rRNA operons and their flanking sequences, we studied a sample of 1346 strains within the taxon. DNA from each strain was digested with a restriction endonuclease, EcoRI. The fragments were separated by gel electrophoresis, immobilized on a membrane, and hybridized with a labeled rRNA operon from Escherichia coli. The pattern of bands, positions, and intensities of hybridized fragments were electronically captured. Software was used to normalize the band positions relative to standards, scale the signal intensity, and reduce the background so that each strain was reproducibly represented in a data base as a pattern. With these methods, L. monocytogenes was resolved into 50 pattern types differing in the length of at least one polymorphic fragment. Pattern types representing multiple strains were recorded as the mathematical average of the strain patterns. Pattern types were arranged by size polymorphisms of assigned rRNA regions into subsets, which revealed the branching genetic structure of the species. Subtracting the polymorphic variants of a specific assigned region from the pattern types and averaging the types within each subset resulted in red...Continue Reading

References

Feb 1, 1992·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·C JacquetJ Rocourt
Sep 1, 1986·Annales De L'Institut Pasteur. Microbiology·F Grimont, P A Grimont
Mar 8, 1974·Biochimica Et Biophysica Acta·E D VerdlovE I Budowsky

❮ Previous
Next ❯

Citations

Jul 6, 2013·Systematic and Applied Microbiology·Peter Schumann, Rüdiger Pukall
Feb 15, 2002·International Journal of Food Microbiology·Maija-Liisa SuihkoPatrick Gustavsson
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·R J HubnerJ A Webster
Aug 10, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C JacquetJ Rocourt
May 2, 2000·Applied and Environmental Microbiology·M WiedmannK J Boor
Jan 3, 2001·Applied and Environmental Microbiology·D M NortonM Wiedmann
Feb 7, 2001·Applied and Environmental Microbiology·M BarneyD Ryder
Feb 7, 2001·Applied and Environmental Microbiology·D M NortonM Wiedmann
Oct 7, 2004·Applied and Environmental Microbiology·Michael J GrayMartin Wiedmann
Feb 10, 2004·Applied and Environmental Microbiology·Wei ZhangStephen J Knabel
Jul 9, 2004·Applied and Environmental Microbiology·J JohnsonA D Hitchins
Jun 28, 2001·Journal of Clinical Microbiology·C A NadonM Wiedmann
Aug 31, 2002·Journal of Clinical Microbiology·Steven CaiMartin Wiedmann
Aug 7, 2004·Journal of Clinical Microbiology·Michel DoumithPaul Martin
Feb 28, 2007·The Journal of General and Applied Microbiology·Ida RomanoAssunta Giordano
Jun 5, 2003·Emerging Infectious Diseases·Brian D SaudersMartin Wiedmann
Jul 17, 2007·International Journal of Food Microbiology·Alessandra De CesareGerardo Manfreda
Sep 11, 2004·Journal of Applied Microbiology·K SuzukiH Yamashita
Jan 1, 1997·Journal of Dairy Science·C A Batt
Sep 13, 2017·International Journal of Systematic and Evolutionary Microbiology·Yuki AsakawaMasayuki Aizawa
Mar 4, 2006·Microbiology·Angela RobertsMartin Wiedmann
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·Koji SuzukiHiroshi Yamashita
May 9, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·R MagalhaesP Teixeira
Sep 18, 2002·Journal of Food Protection·W Payton PruettBonnie Rose
Oct 21, 2000·Journal of Food Protection·G E SkinnerJ Ulaszek

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.