Several glutathione S-transferase isozymes that protect against oxidative injury are expressed in human liver mitochondria

Biochemical Pharmacology
Evan P GallagherDavid S Barber

Abstract

The mitochondrial environment is rich in reactive oxygen species (ROS) that may ultimately peroxidize membrane proteins and generate unsaturated aldehydes such as 4-hydroxy-2-nonenal (4HNE). We had previously demonstrated the presence of hGSTA4-4, an efficient catalyst of 4HNE detoxification, in human liver mitochondria to the exclusion of the cytosol. In the present study, GSH-affinity chromatography was used in conjunction with biochemical and proteomic analysis to determine the presence of additional cytosolic glutathione S-transferases (GSTs) in human hepatic mitochondria. HPLC-subunit analysis of GSH affinity-purified liver mitochondrial proteins indicated the presence of several potential mitochondrial GST isoforms. Electrospray ionization-mass spectrometry analysis of eluted mitochondrial GST subunits yielded molecular masses similar to those of hGSTP1, hGSTA1 and hGSTA2. Octagonal matrix-assisted laser desorption/ionization time of flight mass spectrometry and proteomics analysis using MS-FIT confirmed the presence of these three GST subunits in mitochondria, and HPLC analysis indicated that the relative contents of the mitochondrial GST subunits were hGSTA1>hGSTA2>hGSTP1. The mitochondrial localization of the alpha and...Continue Reading

References

Aug 1, 1977·Biochemical Pharmacology·C N StathamJ J Lech
Dec 1, 1991·Gut·P C HayesD J Harrison
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Sep 26, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alexandra Henrion-CaudeAnnick Clement
Mar 21, 2003·The Journal of Biological Chemistry·Marie-Anne RobinNarayan G Avadhani
May 2, 2003·The Biochemical Journal·Ian R JowseyJohn D Hayes
May 4, 2004·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Shahrokh Mohammadzadeh GhobadlooMohammad Reza Zali
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·John D HayesIan R Jowsey
Sep 8, 2005·Bioinformatics·Chittibabu Guda, Shankar Subramaniam

❮ Previous
Next ❯

Citations

Oct 5, 2010·The Journal of Pharmacology and Experimental Therapeutics·Wenjun LiPeter W Stacpoole
Oct 6, 2015·Biomolecules·Rudolf J SchaurPeter M Eckl
Mar 10, 2009·Free Radical Biology & Medicine·Shinji GotoTakahito Kondo
Apr 9, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xinping TanSatdarshan P S Monga
Jun 5, 2007·Clinics in Liver Disease·Ancha BaranovaZobair M Younossi
Jun 16, 2007·Pharmacology & Therapeutics·Elizabeth M Ellis
May 12, 2009·Biochemical Pharmacology·Jofre Tenorio-LarangaJ Arturo García-Horsman
Nov 21, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Emi AkizawaOsamu Koiwai
Jan 18, 2011·Proteomics·Elisabetta GianazzaMaria Antonietta Vanoni
Nov 6, 2012·Biochimica Et Biophysica Acta·Montserrat MaríJosé C Fernández-Checa
Apr 1, 2010·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Imran AliBhavtosh Sharma
Dec 6, 2011·Mitochondrion·Gerassimos LascaratosAnthony H V Schapira
Jul 16, 2014·Frontiers in Pharmacology·Vicent RibasJosé C Fernández-Checa
Mar 10, 2015·Biochemistry. Biokhimii︠a︡·E V KalininaM D Novichkova
Dec 7, 2007·Medicinal Research Reviews·G PoliG Leonarduzzi
Sep 9, 2009·Fundamental & Clinical Pharmacology·Magdalena DudekLidia Włodek
May 17, 2019·G3 : Genes - Genomes - Genetics·Ran TianGuang Yang
Jan 17, 2020·Critical Reviews in Toxicology·Patrick E Hanna, M W Anders
Feb 14, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Leon A SokulskyPaul S Foster
Jul 1, 2019·G3 : Genes - Genomes - Genetics·Ran TianGuang Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis