Sevoflurane postconditioning protects against myocardial ischemia/reperfusion injury by restoring autophagic flux via an NO-dependent mechanism

Acta Pharmacologica Sinica
Shigang QiaoHui-Ling Zhang

Abstract

Volatile anesthetics improve postischemic cardiac function and reduce infarction even when administered for only a brief time at the onset of reperfusion. A recent study showed that sevoflurane postconditioning (SPC) attenuated myocardial reperfusion injury, but the underlying mechanisms remain unclear. In this study, we examined the effects of sevoflurane on nitric oxide (NO) release and autophagic flux during the myocardial ischemia/reperfusion (I/R) injury in rats in vivo and ex vivo. Male rats were subjected to 30 min ischemia and 2 h reperfusion in the presence or absence of sevoflurane (1.0 minimum alveolar concentration) during the first 15 min of reperfusion. We found that SPC significantly improved hemodynamic performance after reperfusion, alleviated postischemic myocardial infarction, reduced nicotinamide adenine dinucleotide content loss, and cytochrome c release in heart tissues. Furthermore, SPC significantly increased the phosphorylation of endothelial nitric oxide synthase (NOS) and neuronal nitric oxide synthase, and elevated myocardial NOS activity and NO production. All these effects were abolished by treatment with an NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 10 mg/kg, i.v.). We also observed m...Continue Reading

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Citations

Mar 19, 2019·Oxidative Medicine and Cellular Longevity·Chen-Chen CaiZhen-Lang Lin
Nov 14, 2020·Anesthesia and Analgesia·Carolin TorregrozaRagnar Huhn
Apr 4, 2021·International Journal of Molecular Sciences·Sebastian RothRagnar Huhn
Jul 20, 2021·Journal of Oral Biosciences·Keita KagawaTakuro Sanuki
Sep 4, 2021·Experimental and Therapeutic Medicine·Qiu Qian, Yingxiang Xie

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Methods Mentioned

BETA
transmission electron microscopy

Software Mentioned

Alpha View gel
densitometric
GraphPad Prism

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