Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis

Cancer Management and Research
Haoyi LiYao Yu

Abstract

Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration. Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay was hired to check cell apoptosis. The protein levels of B-cell lymphoma-2 (Bcl-2), BCL2-Associated X (Bax), hexokinase 2 (HK2) and magnesium transporter 1 (MAGT1) in samples were measured by Western blot. The abilities of cell migration and invasion were estimated by transwell assay. Glucose colorimetric assay kit and lactate colorimetric assay kit were used to check glucose consumption and lactate production, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the levels of circular RNA (circRNA) circ_0002755 (also known as the circRNA1656) and microRNA (miR)-628-5p in samples. The interaction between miR-628-5p and circ_0002755 or MAGT1 was predicated by starBase, which was verified by the dual-luciferase reporter assay. Xenograft tumor model was established to explore the biological role of circ_0002755 in vivo. Sev inhibited cell viability, migration, invasion and promoted cell apoptosis, and also reduced...Continue Reading

References

Mar 6, 2007·Expert Opinion on Drug Delivery·Adam N Mamelak, Douglas B Jacoby
Mar 30, 2011·PloS One·Zhuo Fang, Nikolaus Rajewsky
Dec 15, 2012·Cancer Genetics·McKinsey L Goodenberger, Robert B Jenkins
May 8, 2014·Journal of Neuro-oncology·Mohamed A HamzaJohn F DeGroot
Sep 16, 2016·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Liang XueJianning Zhang
Nov 15, 2016·International Journal of Molecular Medicine·Wenbo YiXingang Li
Sep 15, 2017·Journal of the National Cancer Institute·Yibing YangNu Zhang
Feb 9, 2018·Oncotarget·Zhijie XuZhicheng Gong
Mar 14, 2018·Cancer Cell·Annamarie E Allen, Jason W Locasale
Jun 28, 2018·Advances in Experimental Medicine and Biology·Sminu Bose, Anne Le
Jul 4, 2018·Cancer Research·Renjie WangHaiqian Liang
Jul 31, 2018·Biochemical and Biophysical Research Communications·Pengcheng JinOuyang Wang
Mar 26, 2019·Cancer Biomarkers : Section a of Disease Markers·Xi ChenYuanliang Yan
Mar 28, 2019·Pathology Oncology Research : POR·Cao GaoXiao-Feng He
May 1, 2019·Journal of Cellular Biochemistry·Guang WangXiaochuan Sun
May 22, 2019·Journal of Cellular Biochemistry·Peng XieXiaohua Zuo
Aug 7, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Le ZhangYanping Wang
Aug 10, 2019·Nature Reviews. Genetics·Lasse S KristensenJørgen Kjems

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Citations

Apr 16, 2021·OncoTargets and Therapy·Tan LiXin-Hua Liang
Jun 30, 2021·Journal of Biological Research·Jingpeng WangHuihua Han
Aug 31, 2021·Metabolic Brain Disease·Zhitao ZhaoRuiming Gao

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Methods Mentioned

BETA
transfection
Assay
flow cytometry
xenograft

Software Mentioned

GraphPad Prism
ARRIVE

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