PMID: 589699Nov 1, 1977

Sex- and strain-dependent hepatic microsomal ethylmorphine N-demethylation in mice: the roles of type I binding and NADPH-cytochrome P-450 reductase

Chemico-biological Interactions
A P van den BergE Koopman-Kool

Abstract

The roles of type I binding and NADPH-cytochrome P-450 reductase in ethylmorphine demethylation were investigated in two strains of mice, using sex differences in these activities as a tool. In the CPB-SE strain, females metabolize ethylmorphine faster than males. Sex differences in cytochrome P-450 content and endogenous NADPH-cytochrome P-450 reductase activity were too small to account for this. On the other hand, the differences in the magnitudes of type I spectra and ethylmorphine-induced enhancement of cytochrome P-450 reduction were considerable larger than those in the rates of demethylation. All parameters, except endogenous cytochrome P-450 reduction, were modified in a similar way by testosterone pretreatment: in females they were depressed to the male level, whereas in males they remained unchanged. Castration had no effect in females and enhanced the activities in males. The CPB-V strain exhibited little or no sex differences in ethylmorphine demethylation, cytochrome P-450 content and endogenous cytochrome P-450 reduction. Testosterone pretreatment had little or no influence on these activities. Type I binding and reductase stimulation, however, showed sex differences, comparable to those observed in the CPB-SE st...Continue Reading

References

Mar 1, 1975·Journal of Steroid Biochemistry·R W EstabrookJ McCarthy
Jan 1, 1973·Annals of the New York Academy of Sciences·R W EstabrookM Waterman
Nov 1, 1970·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H DiehlV Ullrich
Jul 31, 1968·Annals of the New York Academy of Sciences·E S Vesell
Feb 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·B A WESTFALLS GARB

Citations

Apr 25, 1986·Pharmaceutisch Weekblad. Scientific Edition·M Van der GraaffD D Breimer
Jan 1, 1988·Free Radical Biology & Medicine·P Bellavite
Aug 1, 1985·The Science of the Total Environment·H SinghB Kurelec

Related Concepts

Castration
Cytochrome P-450 Oxygenase
Cytochrome Reductases
Ethylmorphine-N-Demethylase
Mice, Inbred Strains
Microsomes, Liver
Oxidoreductases, N-Demethylating
Plasma Protein Binding Capacity
Sterotate

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