Sex affects N-homocysteinylation at lysine residue 212 of albumin in mice

Scientific Reports
Marta SikoraHieronim Jakubowski

Abstract

The modification of protein lysine residues by the thioester homocysteine (Hcy)-thiolactone has been implicated in cardiovascular and neurodegenerative diseases. However, only a handful of proteins carrying Hcy on specific lysine residues have been identified and quantified in humans or animals. In the present work, we developed a liquid chromatography/mass spectrometry targeted assay, based on multiple reaction monitoring, for quantification of N-Hcy-Lys212 (K212Hcy) and N-Hcy-Lys525 (K525Hcy) sites in serum albumin in mice. Using this assay, we found that female (n = 20) and male (n = 13) Cbs-/- mice had significantly elevated levels of K212Hcy and K525Hcy modifications in serum albumin relative to their female (n = 19) and male (n = 17) Cbs+/- littermates. There was significantly more K212Hcy modification in Cbs-/- males than in Cbs-/- females (5.78 ± 4.21 vs. 3.15 ± 1.38 units, P = 0.023). Higher K212Hcy levels in males than in females were observed also in Cbs+/- mice (2.72 ± 0.81 vs. 1.89 ± 1.07 units, P = 0.008). In contrast, levels of the K525Hcy albumin modification were similar between males and females, both in Cbs-/- and Cbs+/- mice. These findings suggest that the sex-specific K212Hcy modification in albumin might ...Continue Reading

References

Jul 3, 2002·The Journal of Biological Chemistry·Hieronim Jakubowski
Aug 2, 2003·Journal of Inherited Metabolic Disease·S Yap
Jan 1, 2004·The Journal of Biological Chemistry·Rafal Glowacki, Hieronim Jakubowski
Jun 24, 2008·Analytical Biochemistry·Hieronim Jakubowski
Aug 19, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hieronim JakubowskiKevin A Strauss
Nov 7, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sapna GuptaWarren D Kruger
Feb 11, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hieronim JakubowskiDiana M Shih
Nov 17, 2009·The Journal of Pediatrics·Hongying Gan-SchreierGeorg F Hoffmann
Feb 23, 2012·Molecular Genetics and Metabolism·Yung-Hsiu LuDau-Ming Niu
Jun 21, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nassila AkchicheJean-Luc Daval
Feb 13, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carine Bossenmeyer-PouriéBernard Beck
Dec 3, 2013·Amino Acids·Marta SikoraHieronim Jakubowski
Nov 3, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joanna Perła-KajanHieronim Jakubowski
Feb 27, 2018·Amino Acids·Kamila BorowczykHieronim Jakubowski
Aug 23, 2018·Trends in Biochemical Sciences·Andrew M JamesMichael P Murphy
Nov 15, 2018·Physiological Reviews·Hieronim Jakubowski

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Citations

Jun 30, 2019·International Journal of Molecular Sciences·Joanna Perła-Kaján, Hieronim Jakubowski
Dec 16, 2020·Expert Review of Proteomics·Hieronim Jakubowski

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