Sex and depot differences in ex vivo adipose tissue fatty acid storage and glycerol-3-phosphate acyltransferase activity

American Journal of Physiology. Endocrinology and Metabolism
Maria Morgan-BathkeMichael D Jensen

Abstract

Adipose tissue fatty acid storage varies according to sex, adipose tissue depot, and degree of fat gain. However, the mechanism(s) for these variations is not completely understood. We examined whether differences in adipose tissue glycerol-3-phosphate acyltransferase (GPAT) might play a role in these variations. We optimized an enzyme activity assay for total GPAT and GPAT1 activity in human adipose tissue and measured GPAT activity. Omental and subcutaneous adipose tissue was collected from obese and nonobese adults for measures of GPAT and GPAT1 activities, ex vivo palmitate storage, acyl-CoA synthetase (ACS) and diacylglycerol-acyltransferase (DGAT) activities, and CD36 protein. Total GPAT and GPAT1 activities decreased as a function of adipocyte size in both omental (r = -0.71, P = 0.003) and subcutaneous (r = -0.58, P = 0.04) fat. The relative contribution of GPAT1 to total GPAT activity increased as a function of adipocyte size, accounting for up to 60% of GPAT activity in those with the largest adipocytes. We found strong, positive correlations between ACS, GPAT, and DGAT activities for both sexes and depots (r values 0.58-0.91) and between these storage factors and palmitate storage rates into TAG (r values 0.55-0.90)....Continue Reading

References

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Jun 12, 2014·Diabetes·Kazanna C HamesMichael D Jensen

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Citations

May 5, 2016·Adipocyte·Maria Morgan-BathkeMichael D Jensen
Feb 17, 2017·Genes & Development·Chelsea HeplerRana K Gupta
Nov 30, 2016·The Journal of Clinical Endocrinology and Metabolism·Iben R AndersenSøren Nielsen
Aug 17, 2019·The Indian Journal of Medical Research·María D RonquilloEsaú Floriano-Sánchez
Oct 2, 2019·The Journal of Clinical Investigation·Lavanya Vishvanath, Rana K Gupta
Mar 1, 2019·International Journal of Molecular Sciences·Ken KarasawaAtsushi Yamashita
Apr 25, 2019·Journal of Gastroenterology and Hepatology·Irena KasackaWojciech Łebkowski
Jul 13, 2021·The Journal of Clinical Endocrinology and Metabolism·Qingyi JiaMichael D Jensen

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