Sex and the single embryo: early deveiopment in the Mediterranean fruit fly, Ceratitis capitata.

BMC Developmental Biology
Paolo GabrieliGiuliano Gasperi

Abstract

In embryos the maternal-to-zygotic transition (MTZ) integrates post-transcriptional regulation of maternal transcripts with transcriptional activation of the zygotic genome. Although the molecular mechanisms underlying this event are being clarified in Drosophila melanogaster, little is know about the embryogenic processes in other insect species. The recent publication of expressed sequence tags (ESTs) from embryos of the global pest species Ceratitis capitata (medfly) has enabled the investigation of embryogenesis in this species and has allowed a comparison of the embryogenic processes in these two related dipteran species, C. capitata and D. melanogaster, that shared a common ancestor 80-100 mya. Using a novel PCR-based sexing method, which takes advantage of a putative LTR retrotransposon MITE insertion on the medfly Y chromosome, the transcriptomes of individual early male and female embryos were analysed using RT-PCR. This study is focused on two crucial aspects of the onset of embryonic development: sex determination and cellular blastoderm formation. Together with the three known medfly genes (Cctransformer, Cctransformer2 and Ccdoublesex), the expression patterns of other medfly genes that are similar to the D. melano...Continue Reading

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Citations

Jun 30, 2012·Journal of Integrative Neuroscience·Devaki NagayaVisweswaran Navaratnam
Jan 17, 2012·BMC Evolutionary Biology·Charles MullonMax Reuter
Dec 5, 2014·BMC Genetics·Jennifer L MorrowMarianne Frommer
Nov 10, 2012·Insect Biochemistry and Molecular Biology·Christian E OgaugwuErnst A Wimmer
Apr 9, 2011·Current Opinion in Genetics & Development·Helen K Salz
Jun 1, 2016·Journal of Biosciences·Suresh Kumar SawanthKallare P Arunkumar

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Datasets Mentioned

BETA
AF115330
GU122240
GU122237
EU437408

Methods Mentioned

BETA
PCR
reverse transcription PCR
electrophoresis

Software Mentioned

BLASTX
flybase
BLAST
Primer3
BLASTN
RNA
fold

Related Concepts

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