Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements

Frontiers in Genetics
Kaleb Pretto GattoLuciana Bolsoni Lourenço

Abstract

The genus Pseudis comprises six frogs of the family Hylidae and only P. tocantins had heteromorphic sex chromosomes detected by classical cytogenetics. In this species, the W chromosome is larger than the Z chromosome and has a large heterochromatic block located between the centromere and the nucleolus organizer region (NOR) in the long arm. This large heterochromatic band is enriched for the PcP190 satellite DNA (satDNA), whereas the Z chromosome bears a smaller C-band adjacent to the centromere in the long arm that is not detected by PcP190 probes. To assess sex chromosome differentiation in the genus Pseudis, we investigated the PcP190 satDNA in P. bolbodactyla, P. cardosoi, P. minuta, and P. paradoxa and in one species of Lysapsus, which is the sister genus of Pseudis. PcP190 sequences were isolated, sequenced, and the diversity of this class of satDNA was analyzed. To evaluate whether sex-related variations in PcP190 satDNA were present, we used in situ hybridization (for P. bolbodactyla, P. paradoxa, P. cardosoi, and P. minuta) and Southern blotting analysis (for all species). We found a low level of sex chromosome heteromorphism in P. bolbodactyla, as a PcP190 cluster was detected in the short arm of one of the homologs...Continue Reading

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Citations

Mar 9, 2020·Cytogenetic and Genome Research·Marcelo J da SilvaPatricia P Parise-Maltempi
Jun 6, 2020·Heredity·Ana B S M FerrettiDiogo C Cabral-de-Mello
Sep 21, 2020·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Vanessa Bellini BardellaDiogo Cavalcanti Cabral-de-Mello
Apr 17, 2021·Frontiers in Genetics·Diogo C Cabral-de-MelloFrantišek Marec

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Datasets Mentioned

BETA
KX170893.1
KX170895.1
KX170898.1
KX170908.1
KX170911.1
KX170916.1
KX170922.1
KX170924
KX170929.1
KX170930.1

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Adobe Photoshop CS3
Clustal W
BioEdit

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