PMID: 8601341Mar 1, 1996Paper

Sex chromosome loss and non-disjunction in women: analysis of chromosomal segregation in binucleated lymphocytes

Chromosoma
A ZijnoR Crebelli

Abstract

Chromosomal lagging and non-disjunction are the main mechanisms of chromosomal malsegregation at mitosis. To date, the relative importance of these two events in the genesis of spontaneous or induced aneuploidy has not been fully elucidated. A methodology based on in situ hybridization with centromeric probes in binucleated lymphocytes was previously developed to provide some insight into this matter. With this method, both chromosomal loss and non-disjunction can be simultaneously detected by following the distribution of specific chromosomes in the nuclei and micronuclei of binucleated cells. In this study, this approach was used for studying the role of chromosomal loss and non-disjunction in the age-related malsegregation of sex chromosomes in females. For this purpose, cultures of cytokinesis-blocked lymphocytes were established from 12 healthy women ranging in age from 25 to 56. The occurrence of malsegregation of X chromosomes in vitro was estimated in binucleated cells that contained four signals, which originates from the division of normal disomic cells. In this cell population, the frequencies of X chromosome loss and non-disjunction ranged from 0% to 1.69% (mean 0.75%), and from 0.20% to 1.33% (mean 0.57%), respecti...Continue Reading

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Citations

Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Carla CostaJoão Paulo Teixeira
Jul 14, 2012·Journal of Toxicology and Environmental Health. Part a·Carla CostaJoão Paulo Teixeira
Jan 21, 2006·Pharmacogenetics and Genomics·Lucia MiglioreAri Hirvonen
Oct 16, 1999·Mutation Research·J M GauthierR S Wells
Jul 30, 2009·Journal of Applied Genetics·E ZietkiewiczM Witt
Aug 18, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yaxian SongQinghua Shi
Nov 24, 2005·Environmental and Molecular Mutagenesis·Constantinos AndrianopoulosNikos A Demopoulos
Aug 21, 2007·American Journal of Medical Genetics. Part a·Fabio CoppedèLucia Migliore

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