Sex-dependent differences in water homeostasis in wild-type and V-ATPase B1-subunit deficient mice

PloS One
Anil V NairDennis Brown

Abstract

Men tend to dehydrate more than women after prolonged exercise, possibly due to lower water intake and higher perspiration rate. Women are prone to exercise-associated hyponatremia, primarily attributed to the higher water consumption causing hypervolemia. Since aquaporin-2 (AQP2) water channels in the kidney collecting duct (CD) principal cells (PCs) are involved in maintaining water balance, we investigated their role in sex-dependent water homeostasis in wild-type (WT) C57BL/6 mice. Because CD intercalated cells (ICs) may also be involved in water balance, we also assessed the urine concentrating ability of V-ATPase B1 subunit-deficient (Atp6v1b1-/-) mice. Upon 12-hour water deprivation, urine osmolality increased by 59% in WT female mice and by only 28% in males. This difference was abolished in Atp6v1b1-/- mice, in which dehydration induced a ~30% increase in urine osmolarity in both sexes. AQP2 levels were highest in WT females; female Atp6v1b1-/- mice had substantially lower AQP2 expression than WT females, comparable to the low AQP2 levels seen in both Atp6v1b1-/- and WT males. After dehydration, AQP2 relocates towards the PC apical pole, especially in the inner stripe and inner medulla, and to a greater extent in WT fe...Continue Reading

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Citations

Jan 12, 2021·Journal of Diabetes and Its Complications·Federica PianiPetter Bjornstad
Mar 23, 2021·American Journal of Physiology. Renal Physiology·Lama Al-QusairiPaul A Welling
Oct 7, 2021·American Journal of Physiology. Cell Physiology·Diana L Torres-PinzonAlicia A McDonough

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