PMID: 8586344Oct 30, 1995Paper

Sex determination by polymerase chain reaction on mummies discovered at Taklamakan desert in 1912

Forensic Science International : Synergy
Z LinT Ohshima

Abstract

Sex determination was performed by the polymerase chain reaction (PCR) on eight adult mummies and one child mummy which were discovered at Taklamakan desert in 1912 and now belong to the Lüshun Museum in China. Archaeologically, these mummies were humans living in the seventh century, that is, more than 1300 years ago. Putative sex determination was performed based on external morphology for six of the eight adults, but it was impossible for the other two adults and one child mummy due to marked destruction on the external morphology. Hair, muscle and skin samples were then collected from each adult mummy, and skin and rib samples from the child mummy. Forty PCR cycles were performed as follows: denaturation at 94 degrees C for 40 s, annealing at 55 degrees C for 30 s and extension at 72 degrees C for 1 min. The primer and PCR reaction mixture were prepared according to the report by Witt and Erickson (M. Witt and R. P. Erikson, A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction. Hum. Genet., 82 (1989) 271-274)). Two different pairs of primer were used. One was X1, X2 (X1: 5'-AATCATCAAATGGAGATTTG-3'; X2: 5'-GTTCAGCTCTGTGAGTGAAA-3') to flanking the 170 bp fragment of the...Continue Reading

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Citations

Sep 1, 2007·Forensic Science, Medicine, and Pathology·E A M Graham
Sep 25, 2004·Mass Spectrometry Reviews·Kurt VarmuzaRobert M Mader
Sep 30, 2005·Pediatrics International : Official Journal of the Japan Pediatric Society·Keiko WadaKatsuya Hisano
Dec 18, 2018·American Journal of Human Biology : the Official Journal of the Human Biology Council·Cristina AfonsoAssumpció Malgosa
Jul 6, 2020·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·María Teresa Navarro-RomeroMiguel Ángel Moreno-Galeana
May 22, 2004·Journal of the American Academy of Dermatology·Eve Judith Lowenstein

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