Sex determination in 58 bird species and evaluation of CHD gene as a universal molecular marker in bird sexing

Zoo Biology
Milos VucicevicZoran Stanimirovic

Abstract

The aim of this research was to test the CHD gene (Chromo Helicase DNA-binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction (PCR) primers: 2550F/2718R and P2/P8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those (Alopochen aegyptiacus, Ara severus, Aratinga acuticaudata, Bucorvus leadbeateri, Cereopsis novaehollandiae, Columba arquatrix, Corvus corax, C. frugilegus, Cyanoliseus patagonus, Guttera plumifera, Lamprotornis superbus, Milvus milvus, Neophron percnopterus, Ocyphaps lophotes, Podiceps cristatus, and Poicephalus senegalus), it was carried out for the first time using molecular markers and PCR. It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may larg...Continue Reading

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Citations

Mar 20, 2015·Developmental Neurobiology·Christopher R OlsonClaudio V Mello
Jan 12, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Emel ÇakmakC Can Bilgin
Feb 24, 2015·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Josephine M McHugh, Siwo R de Kloet
Jul 1, 2017·Zoo Biology·Reza ValadanMansour Aliabadian
Dec 24, 2018·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·César Francisco Díaz CasanaPedro Jorge Chimoy Effio
Nov 22, 2019·Veterinary World·Medania PurwaningrumAris Haryanto
Mar 6, 2021·PloS One·Mariya P DobrevaArkhat Abzhanov
Aug 7, 2021·Genes·Henriëtte van der Zwan, Rencia van der Sluis

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