Sex determination of Microtus mandarinus mandarinus is independent of Sry gene

Mammalian Genome : Official Journal of the International Mammalian Genome Society
Yanqiu ChenBi-Cai Zhu

Abstract

PCR was performed with primers corresponding to the Sry HMG-box of the mouse and eight Microtus species. Primers for the SALL4 gene and the ZFY/ZFX gene were used as positive controls. None of these sets of primers can amplify any homologous segment of the Sry gene in the genomic DNA of Microtus mandarinus mandarinus, but both can amplify the Sry HMG-box in the male mouse, SALL4 bands, and ZFY/ZFX bands in both male and female M. m. mandarinus and mouse. Southern blotting was also used. We used primers for the mouse Sry HMG-box to amplify the Sry HMG-box of the mouse, Microtus arvalis (Microtus), and Pitymys duodecimcostatus (Microtus). The probes were labeled with digoxigenin using PCR after being sequenced. Southern blots were used to detect the genomic DNA of M. m. mandarinus using alkaline phosphatase detection. The results showed that there was a 3.95-kb-blotting band in positive controls: mouse, Microtus arvalis, and Pitymys duodecimcostatus. However, no homologous sequence of the Sry HMG-box was detected in the genomic DNA of M. m. mandarinus. Therefore, we speculate that the Sry HMG-box of M. m. mandarinus is absent or had a big change; sex determination of M. m. mandarinus is independent of Sry. The sex determination m...Continue Reading

References

Oct 1, 1995·Nature Genetics·W JustE Lyapunova
Oct 1, 1994·Molecular Reproduction and Development·V R Harley, P N Goodfellow
Nov 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J A Graves
Jul 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A SánchezR Díaz de la Guardia
Feb 1, 1996·Current Biology : CB·B Charlesworth
Apr 18, 1998·Human Molecular Genetics·M J MitchellJ A Graves
Jul 15, 1998·The Journal of Experimental Zoology·J A Graves
Jul 25, 1998·Cytogenetics and Cell Genetics·W VogelW Just
Mar 25, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P S WesternA H Sinclair
Oct 12, 1999·Cytogenetics and Cell Genetics·M BullejosR Díaz De La Guardia
Feb 15, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S SutouK Tsuchiya
Jun 6, 2002·Trends in Genetics : TIG·Jennifer A Marshall Graves

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Citations

May 6, 2010·Genome Biology·Jennifer A Marshall Graves, Catherine L Peichel
May 20, 2008·Yi chuan = Hereditas·Xin-Rui ZhangBi-Cai Zhu
Apr 10, 2009·Molecular Reproduction and Development·Yufeng WangNan Yan
Jul 10, 2009·Yi chuan = Hereditas·Nan YanYu-Feng Wang
Aug 17, 2016·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·Pietro ParmaEric Pailhoux
Apr 23, 2021·The Journal of Heredity·Scott William Roy

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