Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts.

Proceedings of the National Academy of Sciences of the United States of America
P Bermejo-AlvarezA Gutierrez-Adan

Abstract

Although genetically identical for autosomal Chrs (Chr), male and female preimplantation embryos could display sex-specific transcriptional regulation. To illustrate sex-specific differences at the mRNA level, we compared gene-expression patterns between male and female blastocysts by DNA microarray comparison of nine groups of 60 bovine in vitro-produced blastocysts of each sex. Almost one-third of the transcripts detected showed sexual dimorphism (2,921 transcripts; false-discovery rate, P < 0.05), suggesting that in the absence of hormonal influences, the sex Chrs impose an extensive transcriptional regulation upon autosomal genes. Six genes were analyzed by qPCR in in vivo-derived embryos, which displayed similar sexual dimorphism. Ontology analysis suggested a higher global transcriptional level in females and a more active protein metabolism in males. A gene homolog to an X-linked gene involved in network interactions during spliceosome assembly was found in the Y-Chr. Most of the X-linked-expressed transcripts (88.5%) were up-regulated in females, but most of them (70%) exhibited fold-changes lower than 1.6, suggesting that X-Chr inactivation is partially achieved at the blastocyst stage. Almost half of the transcripts u...Continue Reading

References

Apr 1, 1992·Molecular Reproduction and Development·K P XuK J Betteridge
Mar 1, 1996·Journal of Reproduction and Fertility·K Bredbacka, P Bredbacka
Feb 1, 1997·Molecular and Cellular Biology·A NabetaniT Mukai
Apr 9, 1998·JAMA : the Journal of the American Medical Association·D L DavisJ R Stampnitzky
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·M A LarsonR M Roberts
Feb 9, 2002·Reproduction, Fertility, and Development·A Gutiérrez-AdánJ De La Fuente
Jan 24, 2004·Science·J J EmersonManyuan Long
Jan 31, 2004·Science·Winifred MakNeil Brockdorff
Apr 12, 2005·Neuroscience and Biobehavioral Reviews·William DaviesLawrence S Wilkinson
Oct 26, 2005·Nature Genetics·Ilona ZvetkovaNeil Brockdorff
Mar 15, 2006·Cell·Jennifer A Marshall Graves
Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·A Gutiérrez-AdánD Rizos
Nov 8, 2007·Physiological Genomics·P Bermejo-AlvarezA Gutierrez-Adan
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kevin D SinclairLorraine E Young
Jun 27, 2008·Biology of Reproduction·P Bermejo-AlvarezA Gutiérrez-Adán
Aug 2, 2008·Reproduction, Fertility, and Development·Dimitrios RizosPatrick Lonergan
Nov 20, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Raúl Fernández-GonzálezAlfonso Gutiérrez-Adán
Feb 5, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carlos PenalozaZahra Zakeri
Mar 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Catherine PatratEdith Heard
Jan 6, 2010·Reproduction, Fertility, and Development·Pablo Bermejo-AlvarezDimitrios Rizos
Jan 9, 2010·Molecular Reproduction and Development·R G SturmeyP Lonergan

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Citations

Feb 6, 2013·Journal of Proteome Research·Enrique GómezMarta Muñoz
Oct 5, 2010·Reproduction, Fertility, and Development·M Garcia-HerrerosP Lonergan
Mar 4, 2011·Reproduction, Fertility, and Development·Hélène JammesPascale Chavatte-Palmer
Dec 19, 2012·Reproduction, Fertility, and Development·R Laguna-BarrazaA Gutierrez-Adan
Dec 7, 2013·Reproduction, Fertility, and Development·M HoelkerD Tesfaye
Feb 9, 2012·Stem Cells and Development·Pablo Bermejo-AlvarezAlfonso Gutierrez-Adan
May 27, 2010·Molecular Human Reproduction·David K GardnerGeorge A Thouas
May 28, 2013·BMC Genomics·James L ChitwoodPablo J Ross
Aug 25, 2011·Endocrinology·Kartik ShankarThomas M Badger
Feb 23, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·P Bermejo-AlvarezA Gutierrez-Adan
Mar 18, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·P Bermejo-AlvarezA Gutierrez-Adan
Sep 14, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Claude Robert
Oct 31, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·V Van HoeckR G Sturmey
Apr 16, 2014·Nature Reviews. Genetics·Xinxian DengChristine M Disteche
Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·John J BromfieldSarah A Robertson
May 13, 2014·Chromosoma·Yuichiro Itoh, Arthur P Arnold
Sep 23, 2014·Trends in Genetics : TIG·William P GilksEdward H Morrow
Aug 28, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Luis Manuel Baldoceda-BaldeonClaude Robert
Oct 31, 2014·Proceedings. Biological Sciences·Mingkwan NipitwattanaphonLaurent Keller
Feb 3, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jacques Balthazart
Oct 29, 2015·Theriogenology·Emmanuelle GhysIsabelle Donnay
Jan 12, 2016·Mechanisms of Development·C S OliveiraL S A Camargo
Jan 31, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Kun TanJianhui Tian
Jan 15, 2013·Fertility and Sterility·David K Gardner, Petra L Wale
Nov 15, 2011·Trends in Genetics : TIG·Arthur P Arnold
Aug 23, 2011·Theriogenology·Alexandra CalleAlfonso Gutierrez-Adan
Feb 22, 2011·Trends in Genetics : TIG·Patrick J Wijchers, Richard J Festenstein
Sep 10, 2015·The Journal of Nutritional Biochemistry·Pilar Rodríguez-RodríguezSilvia M Arribas
Feb 22, 2013·Molecular Reproduction and Development·Gael L M Cagnone, Marc-André Sirard
Jan 31, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Arthur P ArnoldKaren Reue

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