Sex Differences in Gene and Protein Expression After Intracerebral Hemorrhage in Mice

Translational Stroke Research
Yongqiu XieMichael L James

Abstract

Sex dimorphism has been demonstrated after experimental intracerebral hemorrhage (ICH). Decreased mortality and improved neurobehavioral outcomes occur in female compared to male mice after intrastriatal autologous blood or collagenase injection. Sex-specific differences in post-ICH gene and protein expression may provide mechanistic insight into this phenomenon. Ten- to 12-week-old C57BL/6 male (M) and female in high estrous state (HE-F) underwent left intrastriatal collagenase injection. We assessed neurobehavioral outcomes over the first 30 days, hematoma volume and cerebral edema evolution over the first 24 h, and transcriptomic gene and protein expression at pre-selected time points during the acute phase of injury. Genome-wide expression profiling was performed with Affymetrix GeneChip Mouse Genome 2.0 Probes, and proteomics analyses were performed using mass spectroscopy. Sex does not affect hemorrhage evolution, but female sex is associated with improved neurobehavioral recovery after ICH. A total of 7037 probes qualified for our filtering criteria, representing 5382 mapped genes and 256 unmapped genes. Female-unique pathways involved cell development, growth, and proliferation, while male-unique pathways involved molec...Continue Reading

References

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Citations

Jan 4, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Jun YanJiping Tang
Aug 13, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Han XiaoPing Liang
Sep 13, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Yuanjian FangJohn H Zhang
Jan 26, 2021·Frontiers in Pharmacology·Andrea C Diaz DiazChristian Waeber
Jul 1, 2021·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Rong PanKe Jian Liu
Sep 17, 2021·Translational Stroke Research·Sravanthi KoduriGuohua Xi

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Pixmeo
Osirix
Affymetrix Microarray Analysis Suite
Bioconductor
IPA

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