Sex Differences in the Methylome and Transcriptome of the Human Liver and Circulating HDL-Cholesterol Levels

The Journal of Clinical Endocrinology and Metabolism
Sonia García-CalzónCharlotte Ling

Abstract

Epigenetics may contribute to sex-specific differences in human liver metabolism. To study the impact of sex on DNA methylation and gene expression in human liver. Cross-sectional, Kuopio Obesity Surgery Study. We analyzed DNA methylation with the Infinium HumanMethylation450 BeadChip in liver of an obese population (34 males, 61 females). Females had a higher high-density lipoprotein (HDL)-cholesterol levels compared with males. Gene expression was measured with the HumanHT-12 Expression BeadChip in a subset of 42 participants. Females displayed higher average methylation in the X-chromosome, whereas males presented higher methylation in autosomes. We found 9455 CpG sites in the X-chromosome and 33,205 sites in autosomes with significant methylation differences in liver between sexes (q < 0.05). When comparing our findings with published studies, 95% of the sex-specific differences in liver methylation in the X-chromosome were also found in pancreatic islets and brain, and 26 autosomal sites showed sex-specific methylation differences in the liver as well as in other human tissues. Furthermore, this sex-specific methylation profile in liver was associated with hepatic gene expression changes between males and females. Notably,...Continue Reading

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Citations

Dec 27, 2019·European Heart Journal·Ahmed HaiderCatherine Gebhard
Sep 12, 2020·Science·Meritxell OlivaBarbara E Stranger
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Jul 3, 2021·Biomedicines·Lucia MiglioreAndrea Stoccoro
Nov 19, 2021·Biochemical Genetics·Meenu Ghai, Farzeen Kader

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Methods Mentioned

BETA
biopsy
gastric bypass
methylation profiling
PCR
transfection
Assay

Software Mentioned

PyroMark Q96
Easeq
R for
WebGestalt
R

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