PMID: 2101797Jan 1, 1990Paper

Sex differences in the subunits of glutathione-S-transferase isoenzyme from rat and human kidney

Enzyme
L ButeraM Bhargava

Abstract

Glutathione-S-transferase (GST) isoenzymes were purified from cytosolic preparations from kidneys of male and female rats and kidney cortical specimens from 2 male and 1 female human subjects. GST isoenzyme expression was analyzed by SDS-PAGE, measurement of catalytic activities with specific substrates and determination of their subunits by ELISA and Western blotting using specific antibodies. GST from female rat kidneys showed a preponderance of subunits 3 and 4; levels of these isoenzymes were 3-4 times greater in females than in males. Levels of subunits 1 and 2 were 1.5-2 times greater in the male rat kidneys. Additional minor bands at 24 and 22 kD were observed in GST preparations from both male and female rat kidneys while a band at 25.3 kD was observed only in the male rat kidney. These bands did not react with antibodies to GST 1-1, GST 2-2 or GST 3-4. Both male and female human kidney samples contained GST isoenzymes comparable to the near-neutral (25-5 kD) and basic forms (25 kD) of GSTs found in human liver. In addition a 28-kD band was present in GST preparations from both male and female human kidneys. Additional bands at 29 and 25.2 kD were present only in male human kidneys. Both the kidney cytosol and the total...Continue Reading

Citations

Oct 22, 2019·Current Drug Metabolism·Askhi M Valodara, Kaid Johar Sr
Apr 4, 2014·Acta Clinica Belgica·S LuyasuA Dive
Apr 24, 2009·Clinical Pharmacokinetics·Offie P Soldin, Donald R Mattison
Mar 16, 2011·Journal of Biomedicine & Biotechnology·Offie P SoldinDonald R Mattison
Feb 17, 2010·Proceedings·Jennifer L DuewallMichael Emmett
Jul 13, 2000·Critical Care Medicine·G A DempseyC D Scheinkestel
Mar 16, 2019·Clinical Medicine : Journal of the Royal College of Physicians of London·William OsborneJon S Friedland
Jun 12, 1998·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·V RodillaG M Hawksworth
Jan 10, 2021·BMJ Case Reports·Anis Zand IraniHolly Gibbons
May 21, 2021·Advanced Drug Delivery Reviews·Christine M MadlaAbdul W Basit

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