sFasL-mediated induction of neutrophil activation in patients with type 2 diabetes mellitus

PloS One
Sona MargaryanGayane Manukyan

Abstract

Fas/Fas ligand system was shown to be related to insulin resistance and type 2 diabetes mellitus (T2DM). However, the role of soluble Fas ligand (sFasL) in functioning of immune cells in type 2 diabetes mellitus (T2DM) has not been studied yet. The aim of the present study was to determine in vitro effects of sFasL on neutrophil activation and apoptosis. We demonstrate here that sFasL exhibited proinflammatory effect and induced mRNA levels of caspase-1, NF-κB, IL-1β and CD18 expression. At the same time, sFasL induced reactive oxygen species (ROS) production. Activation of caspase-1 activity abolished sFasL-dependent apoptosis, and suppressed Fas expression and mRNA levels of caspase-3 in neutrophils from T2DM patients. Collectively, our findings identify a novel proinflammatory role of sFasL in T2DM neutrophils that is dependent of caspase activity. Thus, sFasL enhances inflammatory response of neutrophils from T2DM patients without increasing apoptosis suggesting its triggering role in T2DM inflammation.

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Citations

Jul 18, 2019·Journal of the European Academy of Dermatology and Venereology : JEADV·K KridinK T Amber
Aug 21, 2019·International Wound Journal·Ariana García-OjalvoGerardo Guillén-Nieto
Aug 15, 2020·Frontiers in Immunology·Gholamreza DaryaborKurosh Kalantar
Mar 7, 2021·International Journal of Molecular Sciences·Shulamit B Wallach-DayanRaphael Breuer
Apr 17, 2021·The Journal of Surgical Research·Jian-Chang LinShun-Bin Wang
Jul 15, 2021·Computers in Biology and Medicine·Syed Aun MuhammadQiYu Zhang
Oct 17, 2021·The International Journal of Biochemistry & Cell Biology·W Coles KeeterElena V Galkina

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Methods Mentioned

BETA
Assay
PCR
flow cytometry
ELISA
FACS

Software Mentioned

FlowJo
Statsoft Statistica

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis