SFC for chiral separations in bioanalysis

Journal of Pharmaceutical and Biomedical Analysis
Lukas C HarpsMaria Kristina Parr

Abstract

This review covers literature investigating methods for enantioselective chromatography using supercritical fluids as mobile phase constituents (SFC) in the field of bioanalysis. It provides an overview on method development and screening approaches published in scientific literature 2014-2018. Chiral stationary phases are used to create a chiral environment that allows for discrimination of enantiomers. Especially polysaccharide-based stationary phases are used in methods of recent investigations. In comparison to HPLC chiral SFC separation provides more selective cavity effects of inclusion-type chiral separation phases. Modifier and additive choices as well as further operating conditions like backpressure, temperature and flow rate are summarized and critically discussed. Further on, observed sample pretreatment and possible detection techniques are presented. SFC hyphenated to mass detection was found of major relevance and is therefore further discussed. Coupling of SFC with different detectors allows for straightforward use in bioanalysis. Interfacing MS detectors is generally performed including a make-up pump. Thus, applied make-up conditions were also reviewed. While most of the chiral separations in HPLC are performe...Continue Reading

Citations

Mar 9, 2019·Electrophoresis·Anca-Elena DascaluEmmanuelle Lipka
Mar 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chiara FanaliSalvatore Fanali
May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert K HofstetterAndreas Link
Jan 22, 2021·Journal of Chromatography. a·Abhijit Tarafder, Larry Miller
Oct 22, 2020·Journal of Chromatography. a·Mohammad Mahdi Moein, Thuy A Tran

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