Sfrp1 attenuates TAC-induced cardiac dysfunction by inhibiting Wnt signaling pathway- mediated myocardial apoptosis in mice

Lipids in Health and Disease
Shuo PanJianming Pei

Abstract

Hemodynamic overload causes cardiac hypertrophy leading to heart failure. Wnt signaling pathway was reported activated in heart failure. Secreted frizzled related protein 1 (Sfrp1) is a suppressor of Wnt signaling activation. The aim of the present study was to investigate the protective effect of Sfrp1 on hemodynamic overload- induced cardiac dysfunction. A mice transverse aortic constriction (TAC)- induced heart failure model was established. A recombinant adeno-associated virus 9 (AAV9) vector was used to deliver Sfrp1 gene into myocardium. Fluorescence and immunohistochemistry staining was used to evaluate the effectiveness of viral vector delivery. Invasive hemodynamic examination was used to evaluate cardiac systolic and diastolic functions. Myocardium apoptosis was detected by TUNEL assay. The expression levels of Sfrp1, β-catenin, caspase3, Bax, Bcl-2 and c-Myc were measured by Western blotting. Increased mean arterial pressure and impaired cardiac function confirmed the establishment of TAC model. Sfrp1 protein expression was effectively increased in myocardium of mice treated with AAV9-Sfrp1 viral vector. The viral vector administration improved both systolic and diastolic cardiac functions by reducing myocardial apop...Continue Reading

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Citations

Feb 18, 2020·Therapeutic Advances in Chronic Disease·Anqing Huang, Yuli Huang
Sep 2, 2020·Bioscience, Biotechnology, and Biochemistry·Jingdong GuoXiongxiang Zhu
Feb 16, 2021·Physiological Reports·Ying-Chang HsuehJose A Gomez
Apr 15, 2021·Journal of the American Heart Association·Jesse GammonsSalvatore Mancarella

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Methods Mentioned

BETA
electrophoresis
X-ray

Software Mentioned

Image J
SPSS

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