SGK1 mediates the hypotonic protective effect against H2O2-induced apoptosis of rat basilar artery smooth muscle cells by inhibiting the FOXO3a/Bim signaling pathway.

Acta Pharmacologica Sinica
Bao-Yi ChenYong-Yuan Guan

Abstract

Serum- and glucocorticoid-inducible kinease-1 (SGK1) is a serine/threonine kinase regulated by hypotonic stimuli, which is involved in regulation of cell cycle and apoptosis. Our previous study shows that activation of volume-regulated Cl- channels (VRCCs) protects rat basilar artery smooth muscle cells (BASMCs) against hydrogen peroxide (H2O2)-induced apoptosis. In the present study, we investigated whether SGK1 was involved in the protective effect of VRCCs in BASMCs. We showed that hypotonic challenge significantly reduced H2O2-induced apoptosis, and increased SGK1 phosphorylation, but did not affect SGK1 protein expression. The protective effect of hypotonic challenge against H2O2-induced apoptosis was mediated through inhibiting mitochondria-dependent apoptotic pathway, evidenced by increased Bcl-2/Bax ratio, stabilizing mitochondrial membrane potential (MMP), decreased cytochrome c release from the mitochondria to the cytoplasm, and inhibition of the activation of caspase-9 and caspase-3. These protective effects of hypotonic challenge against H2O2-induced apoptosis was diminished and enhanced, respectively, by SGK1 knockdown and overexpression. We further revealed that SGK1 activation significantly increased forkhead box...Continue Reading

References

Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S WaldeggerF Lang
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·F LangS Broër
Jun 13, 2002·American Journal of Physiology. Renal Physiology·David J RozanskyDavid Pearce
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ge-Xin WangIlia A Yamboliev
Jul 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bing-e XuMelanie H Cobb
Aug 6, 2005·The Journal of Biological Chemistry·Bing-E XuMelanie H Cobb
Oct 26, 2007·American Journal of Physiology. Renal Physiology·Akiyuki TarunoYoshinori Marunaka
Oct 10, 2008·Journal of Cell Science·Ciaran Richardson, Dario R Alessi
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Jun 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Catarina CatelaNadia Rosenthal
Oct 6, 2010·Growth Factors·Maressa A BruhnKaren E Sheppard
Mar 5, 2011·Apoptosis : an International Journal on Programmed Cell Death·Yan QianYong-Yuan Guan
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nathan D BozeatDayue Darrel Duan
Feb 4, 2012·American Journal of Physiology. Renal Physiology·Nourdine FaresseOlivier Staub
Apr 2, 2013·Archives of Toxicology·Krishnendu SinhaParames C Sil
Jul 8, 2014·Apoptosis : an International Journal on Programmed Cell Death·Jia-Wei ZengYong-Yuan Guan
May 6, 2015·Proceedings of the National Academy of Sciences of the United States of America·Andrea MilenkovicBernhard H F Weber
Jun 3, 2015·World Journal of Gastroenterology : WJG·Jian-An BaiQi-Yun Tang
Jan 8, 2016·Pflügers Archiv : European journal of physiology·Stine F PedersenBernd Nilius
Mar 25, 2016·American Journal of Physiology. Renal Physiology·Lama Al-QusairiOlivier Staub
Jun 9, 2016·European Biophysics Journal : EBJ·Podchanart WanitchakoolKarl Kunzelmann
Apr 25, 2017·Nature Cell Biology·Yanhui ZhangRajan Sah
Aug 5, 2017·Acta Pharmacologica Sinica·Ya-Juan ZhangYong-Yuan Guan
Oct 11, 2017·British Journal of Cancer·Weiwei LiuZhihua Tao
Oct 21, 2017·Molecular Neurobiology·G MorrisB K Puri

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Methods Mentioned

BETA
transfection
Assay
electrophoresis
flow cytometry
fluorescence microscopy

Software Mentioned

GraphPad Prism
MetaFluor Imaging
GraphPad

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