SGK1 regulation by miR-466g in cortical collecting duct cells

American Journal of Physiology. Renal Physiology
Mollie E JacobsAlan C Pao

Abstract

Micro-RNAs (miRNAs) are noncoding RNAs that bind target mRNA transcripts and modulate gene expression. In the cortical collecting duct (CCD), aldosterone stimulates the expression of genes that increase activity of the epithelial sodium channel (ENaC); in the early phase of aldosterone induction, one such gene is serum and glucocorticoid regulated kinase 1 (SGK1). We hypothesized that aldosterone regulates the expression of miRNAs in the early phase of induction to control the expression of target genes that stimulate ENaC activity. We treated mpkCCDc14 cells with aldosterone or vehicle for 1 h and used a miRNA microarray to analyze differential miRNA expression. We identified miR-466g as a miRNA that decreased by 57% after 1 h of aldosterone treatment. Moreover, we identified a putative miR-466g binding site in the 3'-untranslated region of SGK1. We constructed an SGK1 3'-untranslated region luciferase reporter and found that cotransfection of miR-466g suppressed luciferase activity in human embryonic kidney-293 cells in a dose-dependent manner. Deletion or introduction of point mutations that disrupt the miR-466g target site attenuated miR-466g-directed suppression of luciferase activity. Finally, we generated stably transduc...Continue Reading

References

Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S Y ChenD Pearce
Jun 8, 1999·The Journal of Biological Chemistry·A Náray-Fejes-TóthG Fejes-Tóth
Mar 16, 2001·American Journal of Physiology. Renal Physiology·J LoffingF Verrey
Nov 7, 2001·The Journal of Biological Chemistry·Peter M SnyderBrittany C Thomas
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Diego Alvarez de la Rosa, Cecilia M Canessa
Nov 14, 2002·American Journal of Physiology. Renal Physiology·My N HelmsAniko Naray-Fejes-Toth
Sep 29, 2004·The Journal of General Physiology·Diego Alvarez de la RosaCecilia M Canessa
Feb 8, 2006·Annual Review of Physiology·Johannes LoffingOlivier Staub
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kristin K KnightPeter M Snyder
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xu-Bao ShiRalph W deVere White
Apr 4, 2008·American Journal of Physiology. Renal Physiology·Geza Fejes-TóthLawrence G Palmer
Aug 13, 2008·Current Opinion in Nephrology and Hypertension·Chou-Long HuangShih-Hua Lin
Aug 22, 2008·Biology of Reproduction·Stephanie D FiedlerLane K Christenson
Apr 9, 2010·Kidney International·Paul A WellingJames B Wade
May 22, 2010·Pflügers Archiv : European journal of physiology·Shinichi Uchida
Jul 14, 2010·The Journal of Biological Chemistry·Rama SoundararajanThomas R Kleyman
Sep 4, 2010·Journal of the American Society of Nephrology : JASN·Emilie Elvira-MatelotXavier Jeunemaitre
Jan 21, 2011·Physiological Reviews·James A McCormick, David H Ellison
May 14, 2011·Journal of the American Society of Nephrology : JASN·Dao-Hong LinWen-Hui Wang
Jul 26, 2011·The Journal of Biological Chemistry·Sheela V ThomasAlan C Pao
Sep 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Grace X Y ZhengPhillip A Sharp
Feb 4, 2012·American Journal of Physiology. Renal Physiology·Nourdine FaresseOlivier Staub
Jun 14, 2012·Current Opinion in Nephrology and Hypertension·Alan C Pao
Mar 19, 2014·Life Sciences·Mollie E JacobsBrian D Cain
Apr 20, 2014·Journal of the American Society of Nephrology : JASN·Robert S EdingerMariana Labarca

❮ Previous
Next ❯

Citations

Aug 16, 2016·International Journal of Molecular Sciences·Yiyun LouFan Jin
Jul 12, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nejla Ozbaki-YaganMichael Bruce Butterworth
Aug 4, 2018·Current Opinion in Nephrology and Hypertension·Michael B Butterworth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Renal Physiology
Jessica A MartelAnikó Náray-Fejes-Tóth
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Guizhu HongJohn Brown
© 2022 Meta ULC. All rights reserved