Sgo1 establishes the centromeric cohesion protection mechanism in G2 before subsequent Bub1-dependent recruitment in mitosis.

Journal of Cell Science
David Perera, Stephen S Taylor

Abstract

Bub1 was one of the first protein kinases identified as a component of the spindle-assembly checkpoint, a surveillance mechanism that delays anaphase onset until all chromosomes are stably attached to spindle microtubules. Whereas the kinase activity of Bub1 is not required for checkpoint function in yeast, its requirement in mammalian cells is still unclear. Using a complementation assay with bona fide BUB1-null mouse embryonic fibroblasts, we show that the kinase activity of Bub1 is not required for checkpoint function or chromosome alignment. Its activity is, however, required for centromeric localisation of Sgo1, a known protector of centromeric cohesion. Despite the absence of Sgo1 from mitotic centromeres in cells devoid of Bub1 activity, centromeric cohesion is still maintained until anaphase. An explanation for this comes from observations showing that Sgo1 is first recruited to centromeric heterochromatin in G2, but then becomes diffusely localised throughout the nucleus in early prophase, before returning to centromeres later in prophase. Importantly, whereas centromeric localisation of Sgo1 in prophase is dependent on the kinase activity of Bub1, its recruitment to centromeric heterochromatin in G2 is not. Rather, th...Continue Reading

References

Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D Röhme
Dec 1, 1994·Molecular and Cellular Biology·B T RobertsM A Hoyt
Sep 11, 2002·Molecular Biology of the Cell·Cheryl D WarrenForrest A Spencer
Mar 17, 2004·Journal of Cell Science·Victoria L JohnsonStephen S Taylor
Aug 11, 2004·The Journal of Cell Biology·Mounia GuenatriGeneviève Almouzni
Dec 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhanyun TangHongtao Yu
Jun 4, 2005·The EMBO Journal·Patrick Meraldi, Peter K Sorger
Jul 28, 2005·Journal of Cell Science·Christopher J MorrowStephen S Taylor
Dec 20, 2005·Current Biology : CB·Sabine VaurJean-Paul Javerzat
Mar 29, 2007·The Journal of Cell Biology·Yekaterina BoyarchukAlexei Arnaoutov
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Oct 11, 2007·Developmental Cell·David PereraStephen S Taylor
Oct 17, 2007·The Journal of Cell Biology·Karthik JeganathanJan M van Deursen
Apr 16, 2008·Developmental Cell·Daniel J Burke, P Todd Stukenberg
Aug 22, 2008·Nature·Yuya YamagishiYoshinori Watanabe
Mar 3, 2009·Current Biology : CB·Barry E McGuinnessKim Nasmyth
Jun 3, 2009·The Journal of Cell Biology·Christiane KlebigPatrick Meraldi

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Citations

Mar 21, 2013·Chromosoma·Hironori Funabiki, David J Wynne
Jan 29, 2011·Cytogenetic and Genome Research·D Clift, A L Marston
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Yixin Yao, Wei Dai
Jan 18, 2014·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Tarik RakkaaChristian Jaulin
Dec 5, 2012·The Journal of Cell Biology·Robin M RickeJan M van Deursen
May 1, 2012·Trends in Genetics : TIG·Cristina Gutiérrez-CaballeroAlberto M Pendás
Mar 1, 2012·Cell Cycle·Robin M Ricke, Jan M van Deursen
Oct 29, 2011·Cell Cycle·Robin M Ricke, Jan M van Deursen
Oct 12, 2013·Current Biology : CB·Jonathan M G Higgins
Nov 24, 2012·Current Biology : CB·Pablo Lara-GonzalezStephen S Taylor
Oct 5, 2010·Trends in Biochemical Sciences·Victor M Bolanos-Garcia, Tom L Blundell
Dec 9, 2014·Nature Communications·Nicola BrownlowPeter J Parker
Dec 3, 2014·Molecular and Cellular Biology·Adele L Marston
Jul 26, 2018·Annual Review of Cell and Developmental Biology·Aniek JanssenGary H Karpen
Jan 30, 2020·Cell Cycle·M Lienhard SchmitzMarkus Seibert
May 7, 2021·Nature Communications·Donghai LiuStanley Nattel
Jul 3, 2021·Seminars in Cell & Developmental Biology·Pablo Lara-GonzalezArshad Desai

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