Sgt1, but not Rar1, is essential for the RB-mediated broad-spectrum resistance to potato late blight.

BMC Plant Biology
Pudota B BhaskarJiming Jiang

Abstract

Late blight is the most serious potato disease world-wide. The most effective and environmentally sound way for controlling late blight is to incorporate natural resistance into potato cultivars. Several late blight resistance genes have been cloned recently. However, there is almost no information available about the resistance pathways mediated by any of those genes. We previously cloned a late blight resistance gene, RB, from a diploid wild potato species Solanum bulbocastanum. Transgenic potato lines containing a single RB gene showed a rate-limiting resistance against all known races of Phytophthora infestans, the late blight pathogen. To better understand the RB-mediated resistance we silenced the potato Rar1 and Sgt1 genes that have been implicated in mediating disease resistance responses against various plant pathogens and pests. The Rar1 and Sgt1 genes of a RB-containing potato clone were silenced using a RNA interference (RNAi)-based approach. All of the silenced potato plants displayed phenotypically normal growth. The late blight resistance of the Rar1 and Sgt1 silenced lines were evaluated by a traditional greenhouse inoculation method and quantified using a GFP-tagged P. infestans strain. The resistance of the Ra...Continue Reading

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Citations

Aug 26, 2010·Plant Physiology·Pudota B BhaskarJiming Jiang
Jul 31, 2013·Plant Physiology·Saowapa DuangpanJiming Jiang
Nov 19, 2008·Annual Review of Plant Biology·Ken Shirasu
Dec 23, 2015·Journal of Agricultural and Food Chemistry·Rogelio SantiagoAna Butrón
May 29, 2013·Molecular Plant Pathology·Jan Rodewald, Bodo Trognitz
May 8, 2010·Molecular Plant Pathology·Anne-Sophie DielenSylvie German-Retana
Mar 31, 2015·Molecular Plant-microbe Interactions : MPMI·Patricia ManosalvaDaniel F Klessig
Mar 11, 2009·Molecular Plant-microbe Interactions : MPMI·Lara C KramerJiming Jiang
Dec 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dong CaoHuaigang Zhang
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Dec 31, 2016·Evolutionary Applications·Hengyou ZhangBao-Hua Song
Mar 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dong CaoBaolong Liu
Dec 10, 2016·Frontiers in Plant Science·Leonardo Galindo-González, Michael K Deyholos
Sep 15, 2020·BMC Bioinformatics·Timofey V IvanisenkoVladimir A Ivanisenko
May 11, 2021·Molecular Plant-microbe Interactions : MPMI·Hari S KarkiDennis A Halterman

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Datasets Mentioned

BETA
US930287

Methods Mentioned

BETA
transgenic
PCR
dissecting

Software Mentioned

ImageJ

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