SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1

Scientific Reports
Zhi-qin LiuShui-lin He

Abstract

PcINF1 was previously found to induce pepper defense response by interacting with SRC2-1, but the underlying mechanism remains uninvestigated. Herein, we describe the involvement of SGT1 in the PcINF1/SRC2-1-induced immunity. SGT1 was observed to be up-regulated by Phytophthora capsici inoculation and synergistically transient overexpression of PcINF1/SRC2-1 in pepper plants. SGT1-silencing compromised HR cell death, blocked H2O2 accumulation, and downregulated HR-associated and hormones-dependent marker genes' expression triggered by PcINF1/SRC2-1 co-overexpression. The interaction between SRC2-1 and SGT1 was found by the yeast two hybrid system and was further confirmed by bimolecular fluorescence complementation and co-immunoprecipitation analyses. The SGT1/SRC2-1 interaction was enhanced by transient overexpression of PcINF1 and Phytophthora capsici inoculation, and SGT1-silencing attenuated PcINF1/SRC2-1 interaction. Additionally, by modulating subcellular localizations of SRC2-1, SGT1, and the interacting complex of SGT1/SRC2-1, it was revealed that exclusive nuclear targeting of the SGT1/SRC2-1 complex blocks immunity triggered by formation of SGT1/SRC2-1, and a translocation of the SGT1/SRC2-1 complex from the plasma me...Continue Reading

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Citations

May 1, 2019·Molecular Plant-microbe Interactions : MPMI·Zhiqin LiuShuilin He
Apr 16, 2019·Frontiers in Plant Science·Zunaira Afzal NaveedGul Shad Ali
Sep 16, 2020·International Journal of Molecular Sciences·Muhammad AliZhen-Hui Gong
Jan 5, 2021·Frontiers in Plant Science·Zunaira Afzal NaveedGul Shad Ali

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Methods Mentioned

BETA
two-hybrid
PCR
Y2H
co-IP
confocal microscopy
two

Software Mentioned

BLASTN

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