Abstract
Apoptosis acts as the primary pathogenesis of cerebral ischemia/reperfusion (I/R) injury. Prior studies have revealed the effects of src homology 2 (SH2)B adaptor protein 1 (SH2B1) in myocardial infarction; however, involvement of SH2B1 in cerebral I/R injury and the underlying mechanisms remain to be investigated. In the present study, neural‑like PC12 cells underwent 6 h of oxygen‑glucose deprivation (OGD) followed by 24 h of reoxygenation (OGD/R). PC12 cells were pre‑transfected with an adenovirus encoding for SH2B1 or GFP prior to exposure to OGD/R. Cell viability, LDH release and the apoptotic cascade were investigated. Reverse transcription‑quantitative polymerase chain reaction and western blotting were employed to analyze mRNA and protein expression levels, respectively. The results of the present study revealed that OGD/R reduced SH2B1 expression in PC12 cells, accompanied by suppressed cell viability and enhanced cell death. Adenovirus‑mediated SH2B1 overexpression, however, resulted in increased viability, reduced LDH release and a reduction in the expression levels of proteins associated with the apoptotic cascade in PC12 cells under the OGD/R condition. A mechanistic explanation may be that the positive effects of ...Continue Reading
References
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 20, 2007·The Journal of Clinical Investigation·Decheng RenLiangyou Rui
Dec 15, 2007·The Journal of Clinical Investigation·Woei-Cherng ShyuHung Li
Sep 19, 2009·Neurotoxicity Research·Venkata Prasuja NakkaRam Raghubir
Sep 13, 2011·Atherosclerosis·Sabrina PrudenteVincenzo Trischitta
Oct 24, 2013·Diabetes·Zheng ChenLiangyou Rui
Apr 1, 2014·Neuroscience·X LiuX Wang
Aug 16, 2014·World Journal of Diabetes·Liangyou Rui
Jun 17, 2015·Cardiovascular Research·Gang WuCongxin Huang
Jun 17, 2015·Cardiovascular Research·Yuichi IkedaIssei Komuro
Mar 11, 2016·Acta Biochimica Et Biophysica Sinica·Xuan LiuShuming Pan
Apr 22, 2016·Cellular and Molecular Neurobiology·Tao TaoXin-Yue Qin
May 8, 2016·Brain Research·Zhihong JianXiaoxing Xiong
May 12, 2016·Journal of Experimental & Clinical Cancer Research : CR·Wei ChenChunfang Zhang
May 25, 2016·Diabetes·Manuel Blandino-RosanoErnesto Bernal-Mizrachi
Nov 2, 2016·Journal of Alzheimer's Disease : JAD·Yijun ShenFude Huang
Dec 14, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hui-Bo WangJian Yang
Feb 12, 2017·Antioxidants & Redox Signaling·Guozhen WuWeidong Zhang
Mar 21, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun-Feng ZhangYing-Fang Tian
Jun 25, 2017·Journal of Neuroinflammation·Haiying ZhangYoung-Guen Kwon