SH2B1 protects against OGD/R‑induced apoptosis in PC12 cells via activation of the JAK2/STAT3 signaling pathway

Molecular Medicine Reports
Jiang YuanYunfu Wang

Abstract

Apoptosis acts as the primary pathogenesis of cerebral ischemia/reperfusion (I/R) injury. Prior studies have revealed the effects of src homology 2 (SH2)B adaptor protein 1 (SH2B1) in myocardial infarction; however, involvement of SH2B1 in cerebral I/R injury and the underlying mechanisms remain to be investigated. In the present study, neural‑like PC12 cells underwent 6 h of oxygen‑glucose deprivation (OGD) followed by 24 h of reoxygenation (OGD/R). PC12 cells were pre‑transfected with an adenovirus encoding for SH2B1 or GFP prior to exposure to OGD/R. Cell viability, LDH release and the apoptotic cascade were investigated. Reverse transcription‑quantitative polymerase chain reaction and western blotting were employed to analyze mRNA and protein expression levels, respectively. The results of the present study revealed that OGD/R reduced SH2B1 expression in PC12 cells, accompanied by suppressed cell viability and enhanced cell death. Adenovirus‑mediated SH2B1 overexpression, however, resulted in increased viability, reduced LDH release and a reduction in the expression levels of proteins associated with the apoptotic cascade in PC12 cells under the OGD/R condition. A mechanistic explanation may be that the positive effects of ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 20, 2007·The Journal of Clinical Investigation·Decheng RenLiangyou Rui
Aug 16, 2014·World Journal of Diabetes·Liangyou Rui
Jun 17, 2015·Cardiovascular Research·Yuichi IkedaIssei Komuro
May 12, 2016·Journal of Experimental & Clinical Cancer Research : CR·Wei ChenChunfang Zhang
May 25, 2016·Diabetes·Manuel Blandino-RosanoErnesto Bernal-Mizrachi
Nov 2, 2016·Journal of Alzheimer's Disease : JAD·Yijun ShenFude Huang
Dec 14, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hui-Bo WangJian Yang
Feb 12, 2017·Antioxidants & Redox Signaling·Guozhen WuWeidong Zhang
Mar 21, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun-Feng ZhangYing-Fang Tian

❮ Previous
Next ❯

Citations

Jun 18, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Laith N Al-EitanMansour A Alghamdi

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
transfection
fluorescence-activated cell sorting
protein assay

Software Mentioned

CytExpert
BandScan
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.