Shakuyaku-kanzo-to inhibits smooth muscle contractions of human pregnant uterine tissue in vitro

The Journal of Obstetrics and Gynaecology Research
Shoko TsujiHideharu Kanzaki

Abstract

Shakuyaku-kanzo-to (SK) is a herbal medicine and is known to possess an antispasmodic effect on skeletal muscle and intestinal smooth muscle. However, it is unclear whether SK is effective in antagonizing uterine smooth muscle contractions. Herein, we investigated the effects of SK on smooth muscle contractions of human pregnant uterine samples. We prepared myometrial strips from uterine tissues of pregnant women who underwent cesarean section for obstetrical indications, and examined the inhibitory effects of SK and its components, shakuyaku (S) and kanzo (K), on agonist-induced and spontaneous contractions in vitro. Oxytocin, prostaglandinF(2α) , and high KCl were utilized as agonists in this study. SK inhibited agonist-induced and spontaneous contractions in a dose-dependent manner. Inhibition of SK on oxytocin-induced contractions occurred at a concentration of 100 µg/mL and reached maximum effect at a concentration of more than 1000 µg/mL. The half max inhibitory concentration of SK was approximately 440 µg/mL in oxytocin-induced contractions. SK at 1000 µg/mL completely inhibited the oxytocin- and prostaglandinF(2α)-induced contractions but not the high KCl-induced contractions. The inhibitory effects on agonist-induced c...Continue Reading

References

Oct 1, 1988·American Journal of Obstetrics and Gynecology·M P SchreyP J Steer
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Feb 16, 2000·Lancet·A Fugh-Berman
May 11, 2006·Nephron. Clinical Practice·Toru HyodoShiro Baba
Aug 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Tsuyoshi NakamotoHideharu Kanzaki
May 17, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Katsuhiko YasudaHiroshi Ozaki
Jul 17, 2008·Internal Medicine·Yoshitaka MaedaTatsuo Shiigai
Apr 25, 2009·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Hiroyuki KinoshitaFumiatsu Yakushiji

Citations

Jan 23, 2014·BioPsychoSocial Medicine·Takakazu OkaJOPM-EBM Working Team
Dec 9, 2015·The Journal of Obstetrics and Gynaecology Research·Mark P Hehir, John J Morrison
Nov 6, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Dongwook KwakChangsu Na
Nov 26, 2014·The Journal of Obstetrics and Gynaecology Research·Genichiro SumiHideharu Kanzaki
Jun 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xue DingYang Yang
Dec 22, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Ho-Sung LeeDae-Yeon Lee

Related Concepts

TJ-68
Drug Combinations
Plant Extracts, Chinese
Licorice
Inotropism
Myometrium
Oxytocic Effect
Antispasmodic Effect
Tocolytic Effect
Peony

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.