Shakuyaku-kanzo-to inhibits smooth muscle contractions of human pregnant uterine tissue in vitro

The Journal of Obstetrics and Gynaecology Research
Shoko TsujiHideharu Kanzaki


Shakuyaku-kanzo-to (SK) is a herbal medicine and is known to possess an antispasmodic effect on skeletal muscle and intestinal smooth muscle. However, it is unclear whether SK is effective in antagonizing uterine smooth muscle contractions. Herein, we investigated the effects of SK on smooth muscle contractions of human pregnant uterine samples. We prepared myometrial strips from uterine tissues of pregnant women who underwent cesarean section for obstetrical indications, and examined the inhibitory effects of SK and its components, shakuyaku (S) and kanzo (K), on agonist-induced and spontaneous contractions in vitro. Oxytocin, prostaglandinF(2α) , and high KCl were utilized as agonists in this study. SK inhibited agonist-induced and spontaneous contractions in a dose-dependent manner. Inhibition of SK on oxytocin-induced contractions occurred at a concentration of 100 µg/mL and reached maximum effect at a concentration of more than 1000 µg/mL. The half max inhibitory concentration of SK was approximately 440 µg/mL in oxytocin-induced contractions. SK at 1000 µg/mL completely inhibited the oxytocin- and prostaglandinF(2α)-induced contractions but not the high KCl-induced contractions. The inhibitory effects on agonist-induced c...Continue Reading


Oct 1, 1988·American Journal of Obstetrics and Gynecology·M P SchreyP J Steer
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Feb 16, 2000·Lancet·A Fugh-Berman
May 11, 2006·Nephron. Clinical Practice·Toru HyodoShiro Baba
Aug 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Tsuyoshi NakamotoHideharu Kanzaki
May 17, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Katsuhiko YasudaHiroshi Ozaki
Jul 17, 2008·Internal Medicine·Yoshitaka MaedaTatsuo Shiigai
Apr 25, 2009·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Hiroyuki KinoshitaFumiatsu Yakushiji


Jan 23, 2014·BioPsychoSocial Medicine·Takakazu OkaJOPM-EBM Working Team
Dec 9, 2015·The Journal of Obstetrics and Gynaecology Research·Mark P Hehir, John J Morrison
Nov 6, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Dongwook KwakChangsu Na
Nov 26, 2014·The Journal of Obstetrics and Gynaecology Research·Genichiro SumiHideharu Kanzaki
Jun 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xue DingYang Yang
Dec 22, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Ho-Sung LeeDae-Yeon Lee

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